Abstract
THE effects of dye solvents on the selectivity of histo-logical staining have not been extensively investigated. Robards and Purvis1 have reported preferential staining of the gelatinous fibres of tension wood by chlorazol black E when dissolved in methyl cellosolve. Earlier the influence of solvents on the staining reactions of this dye was discussed by Cannon2. Puchtler and Sweat3 reported a method for selectively staining haemoglobin in sections with amido black in a methanol solution, but not in water. Investigation of phospholipid staining by Luxol fast blue G revealed a changing selectivity dependent on the solvent used4. This dye in methanol solution was later found to stain only collagen and elastin5.
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References
Robards, A. W., and Purvis, M. J., Stain Tech., 39, 309 (1964).
Cannon, H. G., J. Roy. Micros. Soc., 61, 88 (1941).
Puchtler, H., and Sweat, F., J. Histochem. Cytochem., 12, 900 (1964).
Salthouse, T. N., Nature, 199, 821 (1963).
Salthouse, T. N., J. Histochem. Cytochem., 13, 133 (1965).
Kirschbaum, J. (personal communication).
Tanford, C., Physical Chemistry of Macromolecules (John Wiley and Sons Inc., New York, 1963).
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SALTHOUSE, T. Preferential Staining of Collagen by Acid Dyes in Methanol. Nature 210, 1277 (1966). https://doi.org/10.1038/2101277a0
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DOI: https://doi.org/10.1038/2101277a0
