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Blood Platelets as a Source of Enzyme Activity in Washed Trypanosome Suspensions

Abstract

THE standard method used for separating trypanosomes from infected blood involves repeated differential centrifugation of a suspension of trypanosomes and blood elements in a phosphate-buffered saline solution containing glucose. The success of this procedure varies not only from one species to another but also with the degree of parasitaemia. Suspensions of the Liverpool strain of Trypanosoma rhodesiense may be obtained free of red blood cells and leucocytes in this way, but with T. vivax, T. congolense, T. lewisi and the ‘M’ and ‘J’ strains of T. rhodesiense other techniques have to be used for complete removal of blood cells and platelets1,2.

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References

  1. Grant, P. T., and Fulton, J. D., Biochem. J., 66, 242 (1957).

    Article  CAS  Google Scholar 

  2. Fulton, J. D., and Spooner, D. F., Exp. Parasitol., 8, 137 (1959).

    Article  CAS  Google Scholar 

  3. Cramer, W., and Bannerman, R. G., Lancet, i, 229 (1930).

  4. Tullis, J. L., Blood, 7, 891 (1952).

    CAS  PubMed  Google Scholar 

  5. Garvin, J. E., J. Exp. Med., 114, 51 (1961).

    Article  CAS  Google Scholar 

  6. Johnson, T. M., and Garvin, J. E., Proc. Soc. Exp. Biol. and Med., 102, 333 (1961).

    Article  Google Scholar 

  7. Nordøy, A., and Ødegaard, A. E., Scand. J. Clin. Lab. Invest., 15, 309 (1963).

    Google Scholar 

  8. Born, G. V. R., and Cross, M. J., Nature, 197, 974 (1963).

    Article  ADS  CAS  Google Scholar 

  9. Zücker, H. B., and Borrelli, J., Ann. N.Y. Acad. Sci., 75, 203 (1958).

    Article  ADS  Google Scholar 

  10. Plat, P. M., Bastide, P., and Dastugue, G., Pathol. Biol. Sem. Hôp., 11, 352 (1963).

    CAS  Google Scholar 

  11. Grant, P. T., and Sargent, J. R., Biochem. J., 76, 229 (1960).

    Article  CAS  Google Scholar 

  12. Grant, P. T., and Sargent, J. R., Biochem. J., 81, 206 (1961).

    Article  CAS  Google Scholar 

  13. Grant, P. T., Sargent, J. R., and Ryley, J. F., Biochem. J., 81, 200 (1961).

    Article  CAS  Google Scholar 

  14. Shaw, J. J., Voller, A., and Bryant, C., Ann. Trop. Med. Parasitol., 58, 17 (1964).

    Article  CAS  Google Scholar 

  15. Brown, K. N. (unpublished).

  16. Williamson, J., Exp. Parasitol., 2, 348 (1953).

    Article  CAS  Google Scholar 

Download references

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DIXON, H. Blood Platelets as a Source of Enzyme Activity in Washed Trypanosome Suspensions. Nature 210, 428 (1966). https://doi.org/10.1038/210428a0

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