Abstract
THE standard method used for separating trypanosomes from infected blood involves repeated differential centrifugation of a suspension of trypanosomes and blood elements in a phosphate-buffered saline solution containing glucose. The success of this procedure varies not only from one species to another but also with the degree of parasitaemia. Suspensions of the Liverpool strain of Trypanosoma rhodesiense may be obtained free of red blood cells and leucocytes in this way, but with T. vivax, T. congolense, T. lewisi and the ‘M’ and ‘J’ strains of T. rhodesiense other techniques have to be used for complete removal of blood cells and platelets1,2.
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DIXON, H. Blood Platelets as a Source of Enzyme Activity in Washed Trypanosome Suspensions. Nature 210, 428 (1966). https://doi.org/10.1038/210428a0
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DOI: https://doi.org/10.1038/210428a0
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