Abstract
WHEN a specimen is bombarded with electrons, X-rays are emitted. Each element in the specimen emits its own X-ray spectrum which consists of a number of prominent lines superimposed on a continuous background. The spectral lines emitted by each element are characteristic of the element, in the way that the D doublet (λ = 600 nm) is characteristic of the optical spectrum of sodium, and they can be used to identify the elements which are present in the specimen.
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References
Cosslett, V. E., and Duncomb, P., Nature, 177, 1172 (1956).
Duncomb, P., Brit. J. App. Phys., 11, 169 (1960).
Berube, G. R., Powers, M. M., Kerkay, J., and Clark, G., Stain Technol., 41, 73 (1966).
Pakkenberg, H., J. Histochem. Cytochem., 10, 367 (1962).
Mellors, R. C., Glassman, A., and Papanicolaou, G. M., Cancer, 5, 458 (1952).
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SIMS, R., MARSHALL, D. Location of Nucleic Acids by Electron Probe X-ray Microanalysis. Nature 212, 1359 (1966). https://doi.org/10.1038/2121359a0
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DOI: https://doi.org/10.1038/2121359a0
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