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Nuclear Membrane Hydrolysis of Adenosine Triphosphate

Abstract

ADENOSINE triphosphate (ATP) hydrolysis was studied cytochemically in embryonic myocardia of the chick, using a modification of Wachstein and Meisel's method1. Fresh embryonic hearts were pre-fixed in ice cold 2.5 per cent glutaraldehyde for 30 min and stored at 0°–4° C in an isotonic sucrose medium containing 0.1 molar tris maleate sodium hydroxide buffer at pH. 7.2. After 3 days, tissues less than 0.5 mm3 were pre-incubated in a reaction medium without ATP to allow for ionic equilibration. The medium contained a final concentration of 0.03 molar tris maleate–sodium hydroxide buffer at pH. 7.2, 0.10 molar sodium ions, 0.03 molar potassium chloride and 0.005 molar magnesium chloride, hexahydrate. Lead nitrate, 0.005 molar, was added just before 0.0025 molar ATP (sodium salt) was added. The reaction was run for 30 min at 30° C, both with and without substrate.

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References

  1. Wachstein, M., and Meisel, E., Amer. J. Clin. Path., 27, 13 (1957).

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  2. Siebert, G., Proc. Fifth Internal. Congr. Biochem., Moscow, edit. by Lindberg, O., 2, 93 (Macmillan, New York, 1963).

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  3. Bankowski, Z., Folia Histochem. Cytochem., 1, 17 (1963).

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KLEIN, R., AFZELIUS, B. Nuclear Membrane Hydrolysis of Adenosine Triphosphate. Nature 212, 609 (1966). https://doi.org/10.1038/212609a0

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