Abstract
WE have failed to confirm the report1 that the injection of several hormones into rats is followed by rapid and specific alterations in the profile obtained by counter-current fractionation of “rapidly labelled RNA” from the liver. We consider that the alterations of profile described could have resulted from slight differences in the procedures for isolating RNA from experimental and control rats. In our work, we have used double labelling to eliminate the possibility of such differences: animals treated with hormones received a tritium precursor while control animals received a carbon-14 precursor and the livers were combined before isolation and fractionation of RNA.
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References
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LEADER, D., BARRY, J. Counter-current Distribution of Rapidly Labelled RNA from Rats treated with Hormones. Nature 215, 1374–1375 (1967). https://doi.org/10.1038/2151374a0
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DOI: https://doi.org/10.1038/2151374a0
