Abstract
IT is known that active tyrosinase (o-diphenol: O2 oxidoreductase) occurs in melanotic melanomata; however, its presence in the amelanotic variety of these tumours is still controversial. Tyrosinase activity in pigmented melanomata was detected and investigated by means of the Warburg manometrical method using tyrosine and dihydroxyphenylalanine (DOPA) as substrates for the enzyme1,2. To the best of our knowledge, however, when this method has previously been used to investigate amelanotic melanomata, it has failed to detect any tyrosinase activity3,4. Other methods used for studying these tumours have given disparate results5–10. For this reason we decided to use the manometrical method of Warburg to investigate amelanotic melanomata in hamsters, which have previously been described, on the basis of histo-chemical investigations, as “tyrosine-positive”8.
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DERNAŁOWICZ, E., TROJANOWSKI, J., BOMIRSKI, A. et al. Tyrosinase Activity in the Amelanotic Melanoma in Golden Hamsters. Nature 215, 188–189 (1967). https://doi.org/10.1038/215188a0
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DOI: https://doi.org/10.1038/215188a0
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