Abstract
WE have found that isonicotinic acid hydrazide, a widely used drug1, is a potent inhibitor of the crosslinking of fibrin. This reaction constitutes the terminal step in normal blood coagulation and proceeds by way of an enzyme catalysed transpeptidation by forming inter-fibrin γ-glutamyl-ε-lysine bonds2,3. Compounds which resemble the donor (amine) or the acceptor (carbonyl) side-chain functions of fibrin specifically inhibit the crosslinking reaction without, however, interfering with gel formation—that is, clotting time—itself.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goodman, L. S., and Gilman, A., The Pharmacological Basis of Therapeutics, third ed., 1322 (The Macmillan Co., NY, 1966).
Lorand, L., and Ong, H. H., Biochem. Biophys. Res. Commun., 23, 188 (1966).
Lorand, L., Ong, H. H., Lipinski, B., Rule, N. G., Downey, J., and Jacobsen, A., Biochem. Biophys. Res. Commun., 25, 629 (1966).
Lorand, L., and Jacobsen, A., Biochemistry, 3, 1939 (1964).
Lorand, L., and Ong, H. H., Biochemistry, 5, 1474 (1966).
Lorand, L., and Konishi, K., Arch. Biochem. Biophys., 105, 58 (1964).
Konishi, K., and Lorand, L., Biochim. Biophys. Acta, 121, 177 (1966).
Bruner-Lorand, J., Pilkington, T. R. E., and Lorand, L., Nature, 210, 1273 (1966).
Waelsch, H., and Mycek, M. J., in Methods in Enzymology (edit. by Colowick, S. P., and Kaplan, N. O.), 5, 833 (Academic Press, 1962).
Lorand, L., Doolittle, R. F., Konishi, K., and Riggs, S. K., Arch. Biochem. Biophys., 102, 171 (1963).
Bruner-Lorand, J., Urayama, T., and Lorand, L., Biochem. Biophys. Res.Commun., 23, 828 (1966).
Tipper, D. J., and Strominger, J. L., Proc. US Nat. Acad. Sci., 54, 1133 (1965).
Wise, jun., E. M., and Park, J. T., Proc. US Nat. Acad. Sci., 54, 75 (1965).
Lorand, L., Konishi, K., and Jacobsen, A., Nature, 194, 1148 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LORAND, L., JACOBSEN, A. Isonicotinic Acid Hydrazide as an Inhibitor of Transpeptidation: Relevance for Blood Coagulation. Nature 216, 508–509 (1967). https://doi.org/10.1038/216508b0
Received:
Published:
Issue date:
DOI: https://doi.org/10.1038/216508b0
