Abstract
SINCE Nowell's original description of phytohaemagglutinin (PHA)-induced lymphocyte transformation1, there has been considerable controversy as to whether responses to this substance involve immunoglobulin and specific antibody synthesis. Several workers2–5, using a variety of techniques, claim to have demonstrated immunoglobulin synthesis by PHA-stimulated human peripheral blood lymphocyte cultures, but others have been unable to confirm these findings6–9. Recent studies with the pokeweed mitogen (PWM) suggest that responses to this stimulant differ morphologically from those to PHA (ref. 10) and that a sub-population of cells responding to PWM may synthesize immunoglobulin9,11.
Similar content being viewed by others
Article PDF
References
Nowell, P. C., Cancer Res., 20, 462 (1960).
Hirschhorn, K., Bach, F., Kolodny, R. L., Firschein, I. L., and Hashem, N., Science, 142, 1185 (1963).
Elves, M. W., Roath, S., Taylor, G., and Israels, M. C. G., Lancet, i, 1292 (1963).
Turner, K. J., and Forges, I. J., J. Immunol., 96, 926 (1966).
Ripps, C. S., and Hirschhorn, K., Clin. Exp. Immunol., 2, 377 (1967).
Sell, S., Rowe, D. S., and Gell, P. G. H., J. Exp. Med., 122, 823 (1965).
Epstein, L. B., Fed. Proc., 25, 439 (1966).
Greaves, M. F., in Proc. Symp. The Biological Effects of Phytohaemagglutinin, 53 (edit. by Elves, M. W., Oswestry, 1966).
Greaves, M. F., and Roitt, I. M., Clin. Exp. Immunol., 3, 393 (1968).
Douglas, J. D., Hoffman, P. F., Borjeson, J., and Chessin, L. N., J. Immunol., 98, 17 (1967).
Greaves, M. F., and Roitt, I. M., Lancet, ii, 419 (1967).
Forbes, I. J., Lancet, i, 198 (1965).
Hullinger, L., and Sorkin, E., Nature, 198, 299 (1963).
Tao, T-W., Science, 146, 247 (1964).
Svehag, S. E., Archiv. fuer die gesamte Virusforschung, 15, 261 (1965).
Harris, G., and Littleton, R. J., J. Exp. Med., 124, 621 (1966).
Greaves, M. F., thesis, Univ. London (1968).
Richardson, M., and Dutton, R. W., Science, 146, 655 (1964).
Gengozian, N., and Hubner, K. F., J. Immunol., 99, 184 (1967).
Singhal, S. K., Naspitz, C. K., and Richter, M., Intern. Arch. Allergy, 31, 390 (1967).
Borjeson, J., Reisfield, R., Chessin, L. N., Welsh, P., and Douglas, S. D., J. Exp. Med., 124, 859 (1966).
Ling, N. R., and Holt, P. J. L., J. Cell Sci., 2, 57 (1967).
Jerne, N. K., Nordin, A. A., and Henry, C., in Cell Bound Antibodies, 109 (edit. by Amas, B., and Koprowski, H.) (Wistar Inst. Press, 1963).
Dresser, D. N., and Wortis, H. H., Nature, 208, 859 (1966).
Sterzl, J., and Riha, I., Nature, 208, 858 (1966).
Mishell, R., and Dutton, R. W., J. Exp. Med., 126, 423 (1967).
Meuwissen, H., Bach, F., Vanalten, P., and Good, R. A., Fed. Proc., 26, 477 (1967).
Dukor, P., and Dietrich, F. M., Intern. Arch. Allergy, 32, 521 (1967).
Davies, A. J. S., Festenstein, H., Leuchars, E., Wallis, V. J., and Doenhoff, M. J., Lancet, i, 183 (1968).
Davies, A. J. S., Leuchars, E., and Wallis, V. J., Transplantation, 5, 222 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GREAVES, M., FLAD, HD. Effect of Lymphocyte Stimulants on Specific Antibody Synthesis in vitro. Nature 219, 975–978 (1968). https://doi.org/10.1038/219975a0
Received:
Issue date:
DOI: https://doi.org/10.1038/219975a0
This article is cited by
-
Die Lymphocytenkultur und ihre Beziehung zu klinischen Problemen
Klinische Wochenschrift (1969)
-
Lymphocytenstimulierung durch Pokeweed-Mitogen (PWM)
Klinische Wochenschrift (1969)
