Novel Method for achieving the Cell-free Synthesis of Protein

Abstract

PROTEIN synthesis at the cell-free level is inefficient and short lived in comparison with the same process in vivo. This difference must largely be attributed to the ability of living cells to maintain a constant internal environment. Things are very different in reaction mixtures for cell-free protein synthesis, in which polysomes and an enzyme–tRNA complex are incubated in a buffered solution with amino-acids, GTP and ATP together with an ATP regenerating system. Substrate and product concentrations are undergoing constant and irreversible change until unfavourable conditions develop and the reaction stops.