Abstract
IT is now becoming obvious that the simple view of urease structure and function, current for half a century1, is inadequate. The enzyme is not absolutely specific for urea, and it has at least two additional substrates, hydroxyurea2 and dihydroxyurea3. Kinetic isotope data indicate multiple substrate sites that not only interact but also interconvert4, and multiple molecular forms have been frequently demonstrated by analytic ultracentrifugation and gel filtration techniques5. So far, however, the multiple forms have not been shown to be enzymatically active.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sumner, J. B., J. Biol. Chem., 69, 435 (1926). Sumner, J. B., Graten, N., and Errikson-Quensel, I. B., J. Biol. Chem., 125, 37 (1938). Sumner, J. B., and Poland, L. O., Proc. Soc. Exp. Biol. and Med., 30, 553 (1933).
Fishbein, W. N., Winter, T. S., and Davidson, J. D., J. Biol. Chem., 240, 2402 (1965).
Fishbein, W. N., J. Biol. Chem., 244, 1188 (1969).
Lynn, K. R., and Yankwich, P. E., Biochim. Biophys. Acta, 56, 512 (1962), and 81, 533 (1964).
Reithel, F. J., and Robbins, J. E., Arch. Biochem. Biophys., 120, 158 (1967). Creeth, J. M., and Nichol, L. W., Biochem. J., 77, 230 (1960). Sehgal, P. P., and Naylor, A. W., Plant Physiol., 41, 567 (1966). Hill, R. M., and Elliott, A., Arch. Biochem. Biophys., 113, 405 (1966). Siegel, L. M., and Monty, K. J., Biochem. Biophys. Res. Commun., 19, 494 (1965).
Fishbein, W. N., Symp. V Chromatog. and Electrophor., Brussels, Abstracts, 48–49 (1968), and in the press (1969).
Dounce, A. L., J. Biol. Chem., 140, 307 (1941).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Raymond, S., Ann. NY Acad. Sci., 121, 350 (1964).
Zwaan, J., Analyt. Biochem., 21, 155 (1967). Thorun, W., and Mehl, E., Biochim. Biophys. Acta, 160, 132 (1968).
Hofstee, B. H. J., J. Gen. Physiol., 32, 339 (1949).
Kaplan, N. O., Ann. NY Acad. Sci., 151 382 (1968).
Fishbein, W. N., Ann. NY Acad. Sci. (in the press, 1969).
Blattler, D. P., Contaxis, C. C., and Reithel, F. J., Nature, 216, 274 (1967).
Mamiya, G., and Gorin, G., Biochim. Biophys. Acta, 105, 382 (1965). Conway, E. J., FitzGerald, O., and McGeeney, K., Irish J. Med. Sci., No. 491, 502 (1966).
Kistiakowsky, G. B., and Rosenberg, A. J., J. Amer. Chem. Soc., 74, 5020 (1952). Kistiakowsky, G. B., Mangelsdorf, P. C., Rosenberg, A. J., and Shaw, W. H. R., J. Amer. Chem. Soc., 74, 5015 (1952). Kistiakowsky, G. B., and Shaw, W. H. R., ibid., 75, 2751 (1953).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FISHBEIN, W., SPEARS, C. & SCURZI, W. Spectrum of Urease Isozymes: Genetic, Polymeric and Conformeric. Nature 223, 191–193 (1969). https://doi.org/10.1038/223191a0
Received:
Revised:
Issue date:
DOI: https://doi.org/10.1038/223191a0
