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Transmission of Cytoplasmic Determinants in Amoebae by Micro-injection of RNA-containing Fractions

Nature volume 224, pages 1127–1129 (1969)Cite this article

Abstract

THE techniques of nuclear transfer and micro-injection of cytoplasm have been used in the study of cytoplasmic inheritance in the free-living amoebae Amoeba proteus and Amoeba discoides. It has been shown that certain cellular properties, such as sensitivity to streptomycin and to neomycin, and mean nuclear diameter are influenced by nuclear and self-replicating cytoplasmic hereditary determinants1,2. A. proteus is more sensitive to streptomycin and neomycin and has a greater nuclear diameter than A. discoides. Micro-injection of small quantities of cytoplasm taken from whole A. discoides results in the transfer and expression of the determinant studied in a small fraction of the clones derived from A. proteus which survive the operation1.

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Authors and Affiliations

  1. Department of Zoology, King's College, London

    SHIRLEY E. HAWKINS

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  1. SHIRLEY E. HAWKINS
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HAWKINS, S. Transmission of Cytoplasmic Determinants in Amoebae by Micro-injection of RNA-containing Fractions. Nature 224, 1127–1129 (1969). https://doi.org/10.1038/2241127a0

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