Abstract
By exploiting the natural ability of polynucleotides to align by base pairing and using polynucleotide kinase and ligase, chemically synthesized segments have been combined into a double stranded DNA corresponding to the gene for the earliest characterized tRNA.
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References
Khorana, H. G., Pure Appl. Chem., 17, 349 (1968).
Khorana, H. G., Proc. Seventh Intern. Cong. Biochem., Tokyo, 1967, 17 (1968).
Holley, R. W., Apgar, J., Everett, G. A., Madison, J. T., Marquisee, M., Merrill, S. H., Penswick, J. R., and Zamir, A., Science, 147, 1462 (1965).
Gupta, N. K., Ohtsuka, E., Weber, H., Chang, S. H., and Khorana, H. G., Proc. US Nat. Acad. Sci., 60, 285 (1968).
Gupta, N. K., Ohtsuka, E., Sgaramella, V., Buchi, H., Kumar, A., Weber, H., and Khorana, H. G., Proc. US Nat. Acad. Sci., 60, 1338 (1968).
Gupta, N. K., and Khorana, H. G., Proc. US Nat. Acad. Sci., 61, 215 (1968).
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AGARWAL, K., BÜCHI, H., CARUTHERS, M. et al. Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast . Nature 227, 27–34 (1970). https://doi.org/10.1038/227027a0
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DOI: https://doi.org/10.1038/227027a0
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