Abstract
GLYCIDOL (2,3-epoxypropanol) phosphate inactivates and becomes covalently bound to triose phosphate isomerase (EC 5.3.1.1; TIM); the kinetics and stoichiometry of the reaction, and the structure of the reagent, indicate a specific reaction at the active centre of the enzyme1. The site that is labelled by this inhibitor, and by glycidol in the presence of inorganic phosphate, has now been identified, lodohydroxyacetone phosphate2 and bromohydroxyacetone phosphate3 are also specific inhibitors of TIM. The amino-acid sequence round the site in chicken muscle TIM labelled by the bromo compound has been established4; we were greatly helped by knowing the sequence of their peptide.
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WALEY, S., MILLER, J., ROSE, I. et al. Identification of Site in Triose Phosphate Isomerase Labelled by Glycidol Phosphate. Nature 227, 181 (1970). https://doi.org/10.1038/227181a0
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DOI: https://doi.org/10.1038/227181a0
