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Detection of H-2 Antigens on Mouse Spermatozoa by the Cytotoxicity Test

Abstract

HISTOCOMPATIBILITY antigens of the type H-2 are found on most cells of the mouse, but there are conflicting reports concerning their expression on spermatozoa. Using the indirect immunofluorescence technique, Barth and Russell1 found no H-2 antigen on spermatozoa. Using the techniques of immunogenicity, antibody absorption, and indirect immunofluorescence, Vojtiskova et al.2 concluded that H-2 antigens were probably detectable on spermatozoa by all three procedures. As these authors point out, however, it is difficult to exclude entirely the possibility that contaminating cells were responsible for the positive findings with the first two techniques.

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GOLDBERG, E., AOKI, T., BOYSE, E. et al. Detection of H-2 Antigens on Mouse Spermatozoa by the Cytotoxicity Test. Nature 228, 570–572 (1970). https://doi.org/10.1038/228570a0

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