Abstract
The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.
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COHEN, J., SHRAGER, R., MCNEEL, M. et al. Proton Magnetic Resonance Studies at 220 MHz of the Histidine Residues of Staphylococcal Nuclease. Nature 228, 642–644 (1970). https://doi.org/10.1038/228642a0
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DOI: https://doi.org/10.1038/228642a0
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