Abstract
MITOTIC chromosomes are now being studied by laser micro-irradiation which involves removing small areas (0.5–2 µm long) of either DNA or protein1. The cells are then observed in tissue culture or fixed and stained cytochemically. By these means, it has been possible to examine nucleolus production and function by selectively altering the nucleolar organizer region of chromosomes2. This type of investigation requires only that the cell completes mitosis and survives in interphase long enough for nucleolus formation and function to be assayed. Although much information can be gained from this type of short term experiment, there would be more scope for investigation if irradiated cells could be cultivated for long periods of time, and shown to be capable of undergoing subsequent mitoses.
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References
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BERNS, M., CHENG, W. & HOOVER, G. Cell Division after Laser Microirradiation of Mitotic Chromosomes. Nature 233, 122–123 (1971). https://doi.org/10.1038/233122a0
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DOI: https://doi.org/10.1038/233122a0
