Abstract
TRANSFER of labelled paternal genome has been demonstrated during fertilization in mammals4,5,14,15. The 14C adenine used, however, did not permit precise localization of parental material within the nuclei of the dividing egg. These and other observations using tritiated thymidine (3H-TdR)8 were made on histological sections of eggs, limiting the precision of the localization of the entire paternal DNA complement in egg chromatin. Intense labelling of the germinal cell line in male mice has been described, using 3H-TdR7,11, and we have combined this by using the air-drying method of Tarkowski16 (which gives good preparations of complete egg nuclei) to localize the paternal DNA complement.
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ODARTCHENKO, N., KENEKLIS, T. Localization of Paternal DNA in Interphase Nuclei of Mouse Eggs during Early Cleavage. Nature 241, 528–529 (1973). https://doi.org/10.1038/241528a0
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DOI: https://doi.org/10.1038/241528a0
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