Abstract
WHEN Escherichia coli bacteria deficient in excision repair (uvr) replicate their DNA after ultraviolet irradiation the newly-synthesised DNA contains gaps which are rejoined during subsequent incubation1 by a postreplication repair process believed to be recombinational in nature2. Double mutants (uvr recA) deficient in both excision and post-replication repair are up to 25 times more sensitive to ultraviolet than those deficient in excision alone3. Bacteria deficient in excision repair and DNA polymerase I (uvr polA) in contrast are no more ultraviolet-sensitive than those deficient solely in excision4, which might suggest that DNA polymerase I is not required for successful post-replication repair. The fact that DNA polymerase III seems to be able to substitute for DNA polymerase I in the repair of excision gaps5, and type II DNA strand breaks6 after X irradiation, however, indicates that DNA polymerase I cannot be excluded from involvement in post-replication repair if similar substitution by DNA polymerase III could occur there.
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SEDGWICK, S., BRIDGES, B. Requirement for either DNA polymerase I or DNA polymerase III in post-replication repair in excision-proficient Escherichia coli. Nature 249, 348–349 (1974). https://doi.org/10.1038/249348a0
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DOI: https://doi.org/10.1038/249348a0
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