Abstract
A system to study enzyme evolution experimentally has been developed. Multiple mutations are required to improve enzyme specificity and the authors propose that such mutations will be acceptable only in ‘silent’ gene copies—shown here to be a frequent response to selective pressure.
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Rigby, P., Burleigh, B. & Hartley, B. Gene duplication in experimental enzyme evolution. Nature 251, 200–204 (1974). https://doi.org/10.1038/251200a0
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DOI: https://doi.org/10.1038/251200a0
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