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Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion

Nature volume 252, page 128 (1974)Cite this article

Abstract

INTERCELLULAR adhesion is generally believed to be mediated by specific macromolecular components on the surface of the interacting cells. Soluble factors which may play a part in this process have been identified in a number of systems1–8. We have already described a developmentally-regulated9 carbohydrate binding protein10,11 from the cellular slime mould Dictyostelium discoideum that appears in differentiating amoebae in close correlation with the development of cohesiveness10. This protein is assayed by its erythrocyte agglutination activity that can be inhibited by specific sugars. We now report the isolation of a similar protein from another cellular slime mould, Polysphondylium pallidum that seems to mediate specific cellular adhesion as judged from the following findings: (1) it is present on the cell surface; (2) it is present when the cells are differentiated to a cohesive state but absent when the cells are not cohesive; (3) addition of the purified protein promotes cell cohesion; (4) sugars which react with the active site of the molecule block cell cohesion produced either by the added purified substance or by the endogenous substance present on cohesive cells; (5) the precise reactivities of the protein from P. pallidum differ from the protein from D. discoideum, which correlates with the segregation of these cells in mixed culture.

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Authors and Affiliations

  1. Department of Psychiatry, University of California, San Diego School of Medicine, La Jolla, California, 92037

    STEVEN D. ROSEN, DAVID L. SIMPSON, JED. E. ROSE & SAMUEL H. BARONDES

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  1. STEVEN D. ROSEN
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  2. DAVID L. SIMPSON
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  3. JED. E. ROSE
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  4. SAMUEL H. BARONDES
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ROSEN, S., SIMPSON, D., ROSE, J. et al. Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion. Nature 252, 128 (1974). https://doi.org/10.1038/252128a0

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