Abstract
THE mitogen-induced transformation of resting lymphocytes to proliferating blast forms is associated with a marked increase in amino-acid uptake1, protein synthesis2 and enzyme activities3. There is little information, however, on the effect of transformation on enzymes of amino-acid metabolism. We therefore examined the biosynthetic and degradative enzymes of the non-essential amino acid, proline4, in resting and transformed lymphocytes. We found a selective increase of proline bio-synthetic enzymes in association with transformation. It is interesting that transformation also produced qualitative changes in Δ1-pyrroline-5-carboxylate reductase, the enzyme which catalyses the committed step in proline synthesis.
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VALLE, D., BLAESE, R. & PHANG, J. Increased sensitivity of lymphocyte Δ1pyrroline-5-carboxylate reductase to inhibition by proline with transformation. Nature 253, 214–216 (1975). https://doi.org/10.1038/253214a0
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DOI: https://doi.org/10.1038/253214a0
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