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Suppression of murine virus leukaemogenesis by thioglycollate, a bacteriological culture medium that affects macrophage peroxidase

Nature volume 255, pages 343–344 (1975)Cite this article

Abstract

INFECTION of mice with an oncornavirus such as Friend leukaemia virus (FLV) results in splenomegaly, hepatomegaly, blood cell dyscrasia and eventually death1–3. Such leukaemias can often be prevented or suppressed by either specific immunisation with a vaccine prepared from the virus or leukaemic cells4–5, by treatment with non-specific enhancers of immune responsiveness such as bacterial lipopolysaccharides or polynucleotides6–8, or by an interferon inducer like statalon9, 10. Activated macrophages are thought to be the cells of the reticuloendothelial system primarily involved in nonspecific anti-tumour immunity. Since enhanced metabolic activity of phagocytes has been associated with wide spectrum microbicidal ability, it was interesting to determine whether thioglycollate treatment of mice would stimulate their macrophages and thereby affect FLV-induced leukaemogenesis. In vitro studies revealed that phagocytosis causes stimulation of peroxidase activity and H2O2 production by phagocytic cells from different sources12. It is well established that the combination of peroxidase, H2O2 and a halide such as chloride or iodide exerts a potent antimicrobial affect at acidic pH13–16. We now report marked inhibition of FLV-induced leukaemogenesis in mice injected with thioglycollate; this inhibition could possibly be related to increased peroxidase and glucose-6-phosphate dehydrogenase (G6PD) activities of peritoneal exudate (PE) cell macrophages in the treated animals.

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Authors and Affiliations

  1. Department of Microbiology, Northern Division, Albert Einstein Medical Center, Philadelphia, Pennsylvania, 19141

    ROBERT R. STRAUSS, HERMAN FRIEDMAN, LEONY MILLS & GORDON ZAYON

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  1. ROBERT R. STRAUSS
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  2. HERMAN FRIEDMAN
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  3. LEONY MILLS
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  4. GORDON ZAYON
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STRAUSS, R., FRIEDMAN, H., MILLS, L. et al. Suppression of murine virus leukaemogenesis by thioglycollate, a bacteriological culture medium that affects macrophage peroxidase. Nature 255, 343–344 (1975). https://doi.org/10.1038/255343a0

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