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Influence of antidiuretic hormone on release of lysosomal hydrolase at mucosal surface of epithelial cells from urinary bladder

Nature volume 257, pages 493–495 (1975)Cite this article

Abstract

THE permeability change induced by antidiuretic hormone (ADH) in amphibian urinary bladder is limited to the apical membrane of epithelial cells rich in intracellular secretion granules1. These granules, which may correspond to lysosomes2, are observed to migrate to and fuse with the apical membrane of toad bladder epithelia treated with oxytocin or cyclic AMP3. We have extended these observations and found that ADH elicits a marked, time-dependent increment in the release of lysosomal hydrolases into the extracellular medium of epithelial cells isolated from amphibian bladder. This effect is abolished by specific antiserum to ADH and reduced by preincubation of cells with cortisol. Additional experiments with intact bladder verify that the hydrolases so released occur predominantly at the mucosal surface. Moreover, cortisol antagonises the enhancement of transepithelial water transport and membrane conductance attributable to ADH action.

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Authors and Affiliations

  1. Department of Biology and the Molecular Biology Institute, University of California, Los Angeles, California, 90024

    RICHARD J. PIETRAS, BARBARA J. SEELER & CLARA M. SZEGO

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  1. RICHARD J. PIETRAS
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  2. BARBARA J. SEELER
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  3. CLARA M. SZEGO
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PIETRAS, R., SEELER, B. & SZEGO, C. Influence of antidiuretic hormone on release of lysosomal hydrolase at mucosal surface of epithelial cells from urinary bladder. Nature 257, 493–495 (1975). https://doi.org/10.1038/257493a0

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