Abstract
WE have found that an enzyme, phospolipase C, which has a very specific action in cleaving phosphatidylcholine (lecithin) at its ester linkage (between the α carbon of glycerol and the phosphoryl group of phosphorylcholine) stimulates active Na transport across frog skin in vitro. This action may provide both insight into the sort of processes involved in transepithelial Na transport and a model for the action of proteins induced by aldosterone1,2. This hormone stimulates Na transport across epithelial membranes which, apart from the kidney tubules, include in vitro frog skin, and toad urinary bladder and colon3–7.
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References
Benjamin, W. B., and Singer, I., 186, 269–272 (1974).
Scott, W. N., and Sapirstein, V. S., Proc. natn. Acad. Sci. U.S.A., 72, 4056–4060 (1975).
Crabbé, J., Nature, 200, 787–788 (1963).
Edelman, I. S., Bogoroch, R., and Porter, G. A., Proc. natn. Acad. Sci. U.S.A., 50, 1169–1177 (1963).
Sharp, G. W. G., and Leaf, A., Nature, 202, 1185–1188 (1964).
Crabbé, J., and DeWeer, P., Nature, 202, 298–299 (1964).
Cofré, G., and Crabbé, J., Nature, 207, 1299–1300 (1965).
Eigler, J., Kelter, J., and Renner, E., Klin. Wschr., 14, 737–738 (1967).
Bentley, P. J., J. Physiol., Lond., 195, 317–330 (1968).
Cuthbert, A. W., and Painter, M. E., J. pharm. Pharmac., 23, 262–269 (1971).
Sharp, G. W. G., Coggins, C. H., Lichtenstein, N. S., and Leaf, A., J. clin. Invest., 45, 1640–1647 (1966).
Lichtenstein, N. S., and Leaf, A., J. clin. Invest., 44, 1328–1342 (1965).
Cereijido, M., and Rotunno, C. A., J. gen. Physiol., 51, 280s–289s (1968).
Voûte, C. L., Hanni, S., and Ammann, E., J. Steroid Biochem., 3, 161–165 (1972).
Sapirstein, V. S., and Scott, W. N., Nature, 257, 241–243 (1975).
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YORIO, T., BENTLEY, P. Stimulation of active Na transport by phospholipase C. Nature 261, 722–723 (1976). https://doi.org/10.1038/261722a0
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DOI: https://doi.org/10.1038/261722a0
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