Abstract
TRANSFECTION and molecular hybridisation assays show that animal cells infected with RNA tumour viruses carry DNA copies of the viral genome integrated into their chromosomes (for review see ref. 1). The transfection assay in particular enables the direct demonstration of the biological activity of the viral DNA2–4. Shearing of the DNA5 and fractionation in sucrose gradients6 provided evidence that the virus is specified by DNA pieces of about 6×106. Transfection kinetics5 and transfection probabilities at the endpoint dilution of the DNA1,7 show that one DNA piece of that size is sufficient to accomplish transfection.
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KOPECK, H., HILLOVA, J. & HILL, M. Effect of restriction endonucleases on infectivity of Rous sarcoma virus DNA. Nature 262, 72–74 (1976). https://doi.org/10.1038/262072a0
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DOI: https://doi.org/10.1038/262072a0
