Abstract
THE wide ranging group of plasmids comprising the P compatibility class in Escherichia coli (ref. 1) (the P-1 class in Pseudomonas aeruginosa (ref. 2)) contains within it some members capable of serving as sex factors for the transmission of chromosomal markers from one P. aeruginosa strain to another. Haas and Holloway3 selected variants of one such P-1 plasmid, R68, that had become highly efficient in Pseudomonas chromosomal gene mobilisation. Some of these variants are also efficient sex factors in E. coli and Rhizobium leguminosarum4. Previous work with P-1 plasmids showed that they can incorporate resistance markers from Pseudomonas-specific plasmids and transmit them to E. coli5. We report here the analogous incorporation of the chromosomal trpAB genes from P. aeruginosa into R68.44, and a study of the expression of these genes after transfer to E. coli K12.
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HEDGES, R., JACOB, A. & CRAWFORD, I. Wide ranging plasmid bearing the Pseudomonas aeruginosa tryptophan synthase genes. Nature 267, 283–284 (1977). https://doi.org/10.1038/267283a0
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DOI: https://doi.org/10.1038/267283a0
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