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Amino-terminal fragments of Escherichia coli lac repressor bind to DNA

Nature volume 269, pages 668–672 (1977)Cite this article

Abstract

The N-terminal fragments (residues 1–51 and 1–59) obtained by selective tryptic cleavage of native lac repressor retain the ability to bind DNA. These fragments (headpieces) are monomeric and form complexes which resemble those of tetrameric repressor with non-operator DNA. But, they do not show the high specificity of repressor for operator sequences. The DNA binding has been demonstrated by filter-binding assay as well as in solution using absorption, circular dichroism, and fluorescence measurements.

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Authors and Affiliations

  1. Max-Planck Institut für Biophysikalische Chemie D-34 Göttingen, FRG

    Thomas M. Jovin, Norbert Geisler & Klaus Weber

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  1. Thomas M. Jovin
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  2. Norbert Geisler
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  3. Klaus Weber
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Jovin, T., Geisler, N. & Weber, K. Amino-terminal fragments of Escherichia coli lac repressor bind to DNA. Nature 269, 668–672 (1977). https://doi.org/10.1038/269668a0

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