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Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase

Abstract

The small subunit of the chloroplast enzyme ribulose bisphosphate carboxylase is synthesised as a precursor of higher molecular weight when poly A–mRNA from pea cytoplasmic polysomes is translated by wheat-germ ribosomes. This precursor is taken up into intact isolated chloroplasts, and cleaved to its final size in the absence of protein synthesis. It is deduced that the signal hypothesis does not apply to chloroplasts; an envelope carrier mechanism is proposed.

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Highfield, P., Ellis, R. Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271, 420–424 (1978). https://doi.org/10.1038/271420a0

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