Abstract
The small subunit of the chloroplast enzyme ribulose bisphosphate carboxylase is synthesised as a precursor of higher molecular weight when poly A–mRNA from pea cytoplasmic polysomes is translated by wheat-germ ribosomes. This precursor is taken up into intact isolated chloroplasts, and cleaved to its final size in the absence of protein synthesis. It is deduced that the signal hypothesis does not apply to chloroplasts; an envelope carrier mechanism is proposed.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ellis, R. J. in The Intact Chloroplast (ed. Barber, J.) 335–364 (Elsevier,Amsterdam, 1976).
Bedbrook, J. R. & Bogorad, L. Proc. natn. Acad. Sci. U.S.A. 73, 4309–4313 (1976).
Ellis, R. J., Blair, G. E. & Hartley, M. R. Biochem. Soc. Symp. 38, 137–162 (1973).
Machold, O. & Aurich, O. Biochim. biophys. Acta 281, 103–112 (1972).
Ellis, R. J. Phytochcmistry 14, 89–93 (1975).
Bogorad, L., Davidson, J. N. & Hanson, M. R. in Nucleic Acids and Protein Synthesis in Plants (eds Bogorad, L. & Weil, J. H.) 135–154 (Plenum, New York, 1977).
Kung, S. D., Thornber, J. P. & Wildman, S. G. FEBS Lett. 24, 185–188 (1972).
Ellis, R. J. in Nucleic Acids and Protein Synthesis in Plants (eds Bogorad, L. & Weil, J. H.) 195–212 (Plenum, New York, 1977).
Ellis, R. J. Biochim. biophys. Acta 463, 185–215 (1977).
Ellis, R. J., Highfield, P. E. & Silverthorne, J. Proc. Symp. IVth Int. Cong. Photosynthesis (eds Hall, D. O., Coombs, J. & Goodwin, T. W.) 497–506 (Biochemical Society, London), 1978.
Blair, G. E. & Ellis, R. J. Biochim. biophys. Acta 319, 223–234 (1973).
Ellis, R. J. Comment. Pl. Science (ed. Smith, H.), 31–43 (Pergamon, Oxford, 1976).
Kung, S. D. Science 191, 429–434 (1976).
Wheeler, A. M. & Hartley, M. R. Nature 257, 66–67 (1975).
Gray, J. C. & Kekwick, R. G. O. Eur. J. Biochem. 44, 491–500 (1974).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).
Campbell, P. N. & Blobel, G. FEBS Lett. 72, 215–226 (1976).
Ellis, R. J. & Hartley, M. R. Nature new Biol. 233, 193–196 (1971).
Davies, E., Larkins, B. A. & Knight, R. H. Pl. Physiol. 50, 581–584 (1972).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Roberts, B. E. & Paterson, B. M. Proc. natn. Acad. Sci. U.S.A. 70, 2330–2334 (1973).
Dobberstein, B., Blobel, G. & Chua, N. H. Proc. natn. Acad. Sci. U.S.A. 74, 1082–1085 (1977).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Shore, G. C. & Tata, J. R. Biochim. biophys. Acta 472, 197–236 (1977).
Joy, K. W. & Ellis, R. J. Biochim. biophys. Acta 378, 143–151 (1975).
Brawerman, G. Meth. Enzym. 30, 605–612 (1974).
Neville, D. M. J. biol. Chem. 246, 6328–6334 (1971).
Laemmli, J. K. Nature new Biol. 227, 680–685 (1970).
Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).
Laskey, R. A. & Mills, A. D. Eur. J. Biochem. 56, 335–341 (1975).
Shapiro, D. J. et al. J. biol. Chem. 249, 3665–3671 (1974).
Kessler, S. W. J. Immun. 115, 1617–1624 (1975).
Arnon, D. I. Pl. Physiol. 24, 1–15 (1949).
Burstein, Y. & Schechter, I. Proc. natn. Acad. Sci. U.S.A. 74, 716–720 (1977).
Schatz, G. & Mason, T. L. A. Rev. Biochem. 43, 51–87 (1974).
Poyton, R. D. & Kavanagh, J. Proc. natn. Acad. Sci. U.S.A. 73, 3947–3951 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Highfield, P., Ellis, R. Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271, 420–424 (1978). https://doi.org/10.1038/271420a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/271420a0
This article is cited by
-
Spatial expression of the genome: the signal hypothesis at forty
Nature Reviews Molecular Cell Biology (2011)
-
Isolation and characterization of the rbcS genes from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta) and transient gene expression using the rbcS gene promoter
Fisheries Science (2009)
-
Discoveries in Rubisco (Ribulose 1,5-bisphosphate carboxylase/oxygenase): a historical perspective
Photosynthesis Research (2007)