Abstract
THE human major histocompatibility complex (MHC) is defined by four loci: HLA-A, B and C are serologically defined and HLA-D was initially defined by the mixed lymphocyte reaction (MLR)1. There is now evidence that a group of HLA-linked, serologically defined B cell-specific alloantigens are the gene products of either the D locus itself or of closely linked genes2,3. Strong associations are seen between alleles of HLA-D and the B cell-specific genotypes, and B cell antisera inhibit the MLR4,5. The D-associated antigens differ from the products of HLA-A, B and C in chemical structure and in tissue distribution6. Human B-cell alloantigens resemble Ia antigens in mice. Analysis of the relationships among Ia antigens, MLC loci and other functions mapping in the I region is limited in both humans and mice by the availability of recombinant individuals7. Because such recombinants are rare, we have begun an analysis of this region by mutation8, using immuno-selection to isolate mutants defective for MHC gene products in human lymphoid cell lines. We describe here the first Ia variants to be isolated and characterised in any species.
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References
Bach, F. H. A. Rev. Genet 10, 319–339 (1976).
Van Rood, J., Van Leeuwen, A., Keuning, J. J. & van oud Alblas, A. Tissue Antigens 5, 73–79 (1975).
Solheim, B. G., Bratlie, A., Winther, N. & Thorsby, E. Proceedings of the 6th International Histocompatibility Workshop, 713–18 (1975).
Van Leeuwen, A., Schut, H. R. & Van Rood, J. J. Transpl. Proc. 5, 1539–42 (1973).
Geier, R. & Cresswell, P. Nature 257, 147 (1875).
Humphreys, R. E. et al. J. exp. Med. 144, 98–112 (1976).
Schreffler, D. & David, I. Adv. Immun. 20, 125–195 (1975).
Pious, D., Hawley, P. & Forrest, G. Proc. natn. Acad. Sci. U.S.A. 70, 1397–1400 (1973).
Winchester, R. J. et al. J. exp. Med. 141, 924 (1975).
Bernocco, D. et al. Tissue Antigens (in the press).
Report of 7th International Histocompatibility Workshop, Oxford (in the press).
Pious, D., Soderland, C. & Gladstone, P. Immunogenetics 01 437–448 (1977).
Winchester, R., Wang, C. M., Halper, J. & Hoffman, T. Scand. J. Immun. 5, 745–757 (1976).
Reif, A. E. Immunochemistry 3, 267–278 (1966).
Pious, D., Bodmer, J. & Bodmer, W. Tissue Antigens 4, 247–256 (1974).
Pious, D. & Soderland, C. J. Immun. 113, 1399–1404 (1974).
Pious, D. & Soderland, C. Science 197, 769 (1977).
Kaufman, J., Fuks, A., Giphart, M., Solheim, B. & Strominger, J. L. in Proceedings of the Third IR Gene Workshop (ed. McDevitt, H.) (Academic, New York, in the press).
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GLADSTONE, P., PIOUS, D. Stable variants affecting B cell alloantigens in human lymphoid cells. Nature 271, 459–461 (1978). https://doi.org/10.1038/271459a0
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DOI: https://doi.org/10.1038/271459a0
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