Abstract
The 31P magnetic resonance spectrum of nucleosome cores from calf thymus nuclei has been measured. The spectrum consists of a single symmetric line, with a maximum second component of 5%. It is argued that this reflects a single average state of the DNA backbone, and does not support models that invoke backbone kinking at a frequency of 1 in 10 base pairs.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Weintraub, H., Worcel, A. & Alberts, B. Cell 9, 409–417 (1976).
Sollner-Webb, B. & Felsenfeld, G. Biochemistry 14, 2915–2920 (1975).
Axel, R. Biochemistry 14, 2921–2925 (1975).
Shaw, B. R., Herman, T. M., Kovacic, R. T., Beaudreau, G. S. & Van Holde, K. E. Proc. natn. Acad. Sci. U.S.A. 73, 505–509 (1976).
Lohr, D., Corden, J., Tatchell, K., Kovacic, R. T. & Van Holde, K. E., Proc. natn. Acad. Sci. U.S.A. 74, 79–83 (1977).
Noll, M. & Kornberg, R. D. J. molec. Biol. 109, 393–404 (1977).
Woodcock, C. L. F. J. Cell Biol. 59, 368a (1973).
Olins, A. L. & Olins, O. E. Science 183, 330–332 (1974).
Pardon, J. F. & Wilkins, M. H. F. J. molec. Biol. 68, 115–124 (1972).
Pardon, J. F. et al. Nucleic Acids Res. 2, 2163–2176 (1975).
Baldwin, J. P., Boseley, P. G., Bradbury, M. & Ibel, K. Nature 253, 245–249 (1975).
Finch, J. T. et al. Nature 269, 29–36 (1977).
Noll, M. Nucleic Acids Res. 1, 1573–1578 (1974).
Simpson, R. T. & Whitlock, J. P., Jr Nucleic Acids Res. 3, 117–127 (1976).
Whitlock, J. P., Rushizky, G. W. & Simpson, R. T. J. biol. Chem. 252, 3003–3006 (1977).
Sollner-Webb, B. & Felsenfeld, G. Cell 10, 537–547 (1977).
Griffith, J. D. Science 187, 1202–1203 (1975).
Germond, J. E., Hirt, B., Oudet, P., Gross-Bellard, M. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 72, 1843–1847 (1975).
Crick, F. H. C. & Klug, A. Nature 255, 530–533 (1975).
Sobell, H. M., Tsai, C.-C., Gilbert, S. G., Jain, S. C. & Sakore, T. D. Proc. natn. Acad. Sci. U.S.A. 73, 3068–3072 (1976).
Sobell, H. M., Tsai, C.-C., Jain, S. C. & Gilbert, S. G. J. molec. Biol. 114, 333–365 (1977).
Gorenstein, D. G. J. Am. chem. Soc. 97, 898–900 (1975).
Cozzone, P. & Jardetzky, O. Biochemistry 15, 4860–4865 (1976).
Gorenstein, D. G., Findlay, J. B., Momii, R. K., Luxon, B. A. & Kar, D. Biochemistry 15, 3796–3083 (1976).
Patel, D. Biopolymers 15, 533–558 (1976).
Gueron, M. & Shulman, R. G. Proc. natn. Acad. Sci. U.S.A. 72, 3482–3485 (1975).
Hayashi, F., Akasaka, K. & Hatano, H. Biopolymers 16, 655–677 (1977).
Reinhardt, C. G. & Krugh, T. R. Biochemistry 16, 2890–2895 (1977).
Gorenstein, D. G. & Kar, D. Biochem. biophys. Res. Comm. 65, 1073–1080 (1975).
Kallenbach, N. R. & Berman, H. M. Q. Rev. Biophys. 10, 137–236 (1977).
Marshall, A. J. & Burgoyne, L. A., Nucleic Acid Res. 3, 1101–1110 (1976).
Noll, H. Nature 215, 360–363 (1967).
Laemmli, U. K. Nature 222, 680–685 (1970).
Peacock, A. C. & Dingman, C. W. Biochemistry 7, 668–674 (1968).
Coulter, C. L. & Greaves, M. L. Science 169, 1097–1098 (1970).
Patel, D. J. Biochemistry 13, 2396–2402 (1974).
Carrington, A. & McLachlan, A. D. Introduction to Magnetic Resonance (Harper and Row, London, 1967).
Hanlon, S., Glonek, T. & Chan, A. Biochemistry 15, 3869–3875 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kallenbach, N., Appleby, D. & Bradley, C. 31P magnetic resonance of DNA in nucleosome core particles of chromatin. Nature 272, 134–138 (1978). https://doi.org/10.1038/272134a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/272134a0
