Abstract
THE effects of the fungal metabolite, cytochalasin B, on lymphocyte transformation induced by mitogenic plant Jectins have been much studied1–7. Results have generally been interpreted in terms of the known effects of cytochalasin B on the microfilaments of the cellular cytoskeleton. A potential site of action which has been largely ignored lies in the inhibitory effects of cytochalasin B on metabolite transport. Cytochalasin B is a potent, competitive inhibitor of erythrocyte glucose transport8, and we have shown that it also inhibits thymocyte glucose transport, and that concanavalin A-stimulated glucose transport is more sensitive to this inhibition9. Glucose transport has been shown to be rate-limiting for thymocyte glycolysis10. Also, glucose has been found to be essential for mitogen-stimulated DNA synthesis in lymphocytes11, and we show here that cytochalasin B can exert its inhibitory effect on DNA synthesis by inhibiting glucose transport.
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HUME, D., HANSEN, K., WEIDEMANN, M. et al. Cytochalasin B inhibits lymphocyte transformation through its effects on glucose transport. Nature 272, 359 (1978). https://doi.org/10.1038/272359a0
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DOI: https://doi.org/10.1038/272359a0
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