Abstract
APPLICATIONS of chemically induced dynamic nuclear polarisation (CIDNP) have been limited so far to reactions of small organic molecules1,2. We report here the observation of CIDNP in the 360-MHz NMR spectrum of a protein. The effect is generated by a cyclic reaction of a photo-excited dye with certain amino acid residues that are accessible to the dye. Of the common amino acids polarisation has been observed in tyrosine, histidine and tryptophan. The method distinguishes between exposed and buried groups and thus yields valuable information on the solution structure of proteins. Here we discuss the CIDNP observed in tyrosine residues of bovine pancreatic trypsin inhibitor (BPTI). The three-dimensional structure of BPTI (molecular weight 6,500) has been studied extensively by X-ray crystallography3,4 and by NMR5,6.
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KAPTEIN, R., DIJKSTRA, K. & NICOLAY, K. Laser photo-CIDNP as a surface probe for proteins in solution. Nature 274, 293–294 (1978). https://doi.org/10.1038/274293a0
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DOI: https://doi.org/10.1038/274293a0
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