Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptors

Nature volume 279, pages 387–391 (1979)Cite this article

Abstract

TPA (12-O-tetradecanoyl-phorbol-13-acetate) reversibly inhibits the binding of 125I-labelled epidermal growth factor (EGF) to treated mouse and human cells, but does not affect the binding of various other ligands to their membrane receptors. It alters the affinity of the receptors for EGF without changing the total number of available receptors per cell. Those phorbol esters which stimulate cell growth in culture and have tumour-promoting activity in vivo alter the EGF-receptor affinity, while the biologically inactive derivatives fail to change the affinity of EGF for its receptors.

Access through your institution
Buy or subscribe

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hecker, E. Meth. Cancer Res. 6, 439 (1971).

    CAS  Google Scholar 

  2. Boutwell, R. K. Crit. Rev. Toxicol. 2, 419 (1974).

    Article  CAS  Google Scholar 

  3. Van Duuren, B. L. Am. chem. Soc. Monogrs 173, 24 (1976).

    CAS  Google Scholar 

  4. Slaga, T. J., Sivak, A. & Boutwell, R. K. (eds) Mechanism of Tumour Promotion and Cocarcinogenesis (Raven, New York, 1978).

  5. Hecker, E. Cancer Res. 28, 2338 (1968).

    CAS  PubMed  Google Scholar 

  6. Whitfield, J. F., McManus, J. P. & Gillan, D. J. J. cell. Physiol. 82, 151 (1973).

    Article  CAS  Google Scholar 

  7. Boyton, A. L., Whitfield, J. F. & Issacs, R. J. J. cell. Physiol. 87, 24 (1976).

    Google Scholar 

  8. Sivak, A. J. cell. Physiol. 80, 167 (1972).

    Article  CAS  Google Scholar 

  9. Wigler, M. & Weinstein, I. B. Nature 259, 232 (1976).

    Article  ADS  CAS  Google Scholar 

  10. Yuspa, S. H. et al. Nature 262, 402 (1976).

    Article  ADS  CAS  Google Scholar 

  11. Blumberg, P. M., Driedger, P. E. & Rossow, P. W. Nature 264, 446 (1976).

    Article  ADS  CAS  Google Scholar 

  12. Wenner, C., Hackney, J., Kimelberg, H. & Mayhew, E. Cancer Res. 34, 1731 (1973).

    Google Scholar 

  13. Sivak, A. & Van Duuren, B. L. Chem. Biol. Interactions 3, 401 (1971).

    Article  CAS  Google Scholar 

  14. Sivak, A., Mossman, B. T. & Van Duuren, B. L. Biochem. biophys. Res. Commun. 46, 605 (1972).

    Article  CAS  Google Scholar 

  15. Rohrschneider, L. R., O'Brien, D. H. & Boutwell, R. K. Biochim. biophys. Acta 280, 57 (1972).

    Article  CAS  Google Scholar 

  16. Cohen, S., Carpenter, G. & Lembach, J. J. Adv. metab. Disord. 8, 265 (1975).

    Article  CAS  Google Scholar 

  17. De Larco, J. E. & Todaro, G. J. Proc. natn. Acad. Sci. U.S.A. 75, 4001 (1978).

    Article  ADS  CAS  Google Scholar 

  18. Lee, L. S. & Weinstein, I. B. Science 202, 313 (1978).

    Article  ADS  CAS  Google Scholar 

  19. Todaro, G. J., De Larco, J. E. & Cohen, S. Nature 264, 26 (1976).

    Article  ADS  CAS  Google Scholar 

  20. De Larco, J. E. & Todaro, G. J. J. cell. Physiol. 84, 335 (1978).

    Article  Google Scholar 

  21. Nicolson, G. L., Lacobiere, M. & Eckhart, W. Biochemistry 14, 172 (1975).

    Article  CAS  Google Scholar 

  22. Rechler, M. M., Podskalny, J. M. & Nissley, S. P. Nature 259, 134 (1976).

    Article  ADS  CAS  Google Scholar 

  23. O'Keefe, E., Hollenberg, M. D. & Cuatrecasas, P. Archs Biochem. Biophys. 164, 518 (1978).

    Article  Google Scholar 

  24. Thomophoulos, P., Roth, J., Lovelace, E. & Pastan, I. Cell 8, 417 (1976).

    Article  Google Scholar 

  25. De Larco, J. E. & Todaro, G. J. Cell 8, 365 (1976).

    Article  CAS  Google Scholar 

  26. Langenbach, R. & Kuszynsky, C. J. natn. Cancer Inst. 55, 801 (1975).

    Article  CAS  Google Scholar 

  27. Slaga, T. J., Scribner, J. D., Thompson, S. & Viaje, A. J. natn. Cancer Inst. 57, 1145 (1967).

    Article  Google Scholar 

  28. Schmidt, R. & Hecker, E. Third Meeting Europ. Assoc. Cancer Res. Abstracts, 63 (1978).

  29. Rohrschneider, L. E. & Boutwell, R. K. Nature new Biol. 243, 212 (1973).

    Article  CAS  Google Scholar 

  30. Reynolds, V. H., Boehn, F. H. & Cohen, S. Surg. Forum 16, 108 (1965).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Viral Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205

    Mohammed Shoyab, Joseph E. De Larco & George J. Todaro

Authors
  1. Mohammed Shoyab
    View author publications

    Search author on:PubMed Google Scholar

  2. Joseph E. De Larco
    View author publications

    Search author on:PubMed Google Scholar

  3. George J. Todaro
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shoyab, M., De Larco, J. & Todaro, G. Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptors. Nature 279, 387–391 (1979). https://doi.org/10.1038/279387a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1038/279387a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing