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Unusual location and function of the operator in the Escherichia coli galactose operon

Abstract

The operator of the gal operon is located about 60 base pairs preceding the startpoints of the transcription of the two gal promoters. This location contrasts with the location of the operator in other phage or bacterial operons where the repressor binds more closely to the respective transcription initiation sites. Models explaining how the repressor–operator interactions may control the two gal promoters are presented.

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References

  1. Musso, R. E., di Lauro, R., Adhya, S. & de Crombrugghe, B. Cell 12, 847–854 (1977).

    Article  CAS  Google Scholar 

  2. Buttin, G. J. molec. Biol. 7, 183–205 (1963).

    Article  CAS  Google Scholar 

  3. Adhya, S. & Echols, H. J. Bact. 92, 601–608 (1966).

    CAS  PubMed  Google Scholar 

  4. Parks, J. S., Gottesman, M., Perlman, R. L. & Pastan, I. J. biol. Chem. 246, 2149–2424 (1971).

    Google Scholar 

  5. Nakanishi, S., Adhya, S., Gottesman, M. E. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 70, 334–338 (1973).

    Article  ADS  CAS  Google Scholar 

  6. Gilbert, W. & Maxam, A. Proc. natn. Acad. Sci. U.S.A. 70, 3581–3584 (1973).

    Article  ADS  CAS  Google Scholar 

  7. Bennett, G. N., Schweingruber, M., Brown, K. D., Squires, C. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A 73, 2351–2355 (1976).

    Article  ADS  CAS  Google Scholar 

  8. Maniatis, T. et al. Cell 5, 109–113 (1975).

    Article  CAS  Google Scholar 

  9. Adhya, S. & Miller, W. Nature 279, 492–494 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Buttin, G. J. molec. Biol. 7, 183–205 (1963).

    Article  CAS  Google Scholar 

  11. Sklar, T., Weissman, S., Musso, R., di Lauro, R. & de Crombrugghe, B. J. biol. Chem. 252, 3538–3547 (1977).

    CAS  PubMed  Google Scholar 

  12. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 79, 560–564 (1977).

    Article  ADS  Google Scholar 

  13. Dickson, R. C., Abelson, J. N., Johnson, P., Reznikoff, W. S. & Barnes, W. M. J. molec. Biol. 111, 65–75 (1977).

    Article  CAS  Google Scholar 

  14. Majors, J. J. biol. Chem. (in the press).

  15. Nakanishi, S., Adhya, S., Gottesman, M. E. & Pastan, I. J. biol. Chem. 249, 4050–4056 (1974).

    CAS  PubMed  Google Scholar 

  16. Bennet, G. N. & Yanofsky, C. J. molec. Biol. 121, 179–192 (1978).

    Article  Google Scholar 

  17. Majors, J. Nature 256, 672–679 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Nissley, S. P., Anderson, W. B., Gottesman, M. E., Perlman, R. L. & Pastan, I. J. biol. Chem. 246, 4671–4678 (1971).

    CAS  Google Scholar 

  19. Musso, R., di Lauro, R., Rosenberg, M. & de Crombrugghe, B. Proc. natn. Acad. Sci. U.S.A. 74, 106–110 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  21. Feiss, M., Adhya, S. & Court, D. Genetics 71, 18–26 (1972).

    Google Scholar 

  22. Musso, R. E. et al. Proc. natn. Acad. Sci. U.S.A. 71, 4940–4944 (1974).

    Article  ADS  CAS  Google Scholar 

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DiLauro, R., Taniguchi, T., Musso, R. et al. Unusual location and function of the operator in the Escherichia coli galactose operon. Nature 279, 494–500 (1979). https://doi.org/10.1038/279494a0

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