Abstract
The three mRNAs that encode the capsid proteins of polyoma virus are produced by the excision of different sequences from continuous transcripts of the L strand of viral DNA. All three of the mRNAs have long half lives, and the larger species are not converted to the smaller ones to any measurable extent within the cytoplasm. Therefore the cytoplasmic proportions of late polyoma mRNAs are predetermined by splicing that is confined to the nucleus of the infected cell and which is complete by the time that mRNA is transported to the cytoplasm.
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References
Hsu, M. T. & Ford, J. Proc. natn. Acad. Sci. U.S.A. 74, 4982–4985 (1977).
Dunn, A. R. & Hassell, J. A. Cell 12, 23–26 (1977).
Berget, S. M., Moore, C. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).
Chow, L. T., Gelinas, R. E., Broker, T. R. & Roberts, R. J. Cell 12, 1–8 (1977).
Lavi, S. & Groner, Y. Proc. natn. Acad. Sci. U.S.A. 74, 5323–5327 (1977).
Klessig, D. F. Cell 12, 9–21 (1977).
Haegeman, G. & Fiers, W. Nature 273, 70–73 (1978).
Kourilsky, P., & Chambon, P. Trends Biochem. Sci. 3, 244–247 (1978).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).
Cordell, B., Weiss, S. R., Varmus, H. E. & Bishop, J. M. Cell 15, 79–91 (1978).
Barnett, T. & Rae, P. M. M. Cell 16, 763–775 (1979).
Borst, P. & Grivell, L. A. Cell 15, 705–723 (1978).
Knapp, G., Beckmann, J. S., Johnson, P. R., Fuhrman, S. A. & Abelson, J. Cell 14, 221–236 (1978).
Sakano, H. et al. Nature 277, 627–633 (1979).
Lai, C. J. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 76, 71–75 (1979).
Spector, D. J., McGrogan, M. & Raskas, H. J. J. molec. Biol. 126, 395–414 (1978).
Fraser, N. & ZifT, E. J. molec. Biol. 124, 27–51 (1978).
Ziff, E. & Fraser, N. J. Virol. 25, 897–906 (1978).
Nevins, J. R. & Darnell, J. E. J. Virol. 25, 811–823 (1978).
Siddell, S. & Smith, A. E. J. Virol. 27, 427–435 (1978).
Kamen, R., Favaloro, J. & Parker, J. Cell (in the press).
Favaloro, J., Treisman, R. & Kamen, R. Meth. Enzym. (in the press).
Piper, P. W. J. molec. Biol. 131, 399–407 (1979).
Legon, S., Flavell, A. J., Cowie, A. & Kamen, R. Cell 16, 373–388 (1979).
Lai, C. J., Dhar, R. & Khoury, G. Cell 14, 971–982 (1978).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Veomett, G., Shay, J., Hough, P. V. C. & Prescott, D. M. Methods in Cell Biology, Vol. 13 (ed. Prescott, D. M.), 1–6, Academic, New York (1976).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Levis, R. & Penman, S. Cell 11, 105–113 (1977).
Seif, I., Khoury, G. & Dhar, R. Nucleic Acids Res. (in the press).
Soeda, E., Arrand, J. R. & Griffin, B. E. Cell (in the press).
Tsai, M. J., Tsai, S. Y. & O'Malley, B. W. Science 204, 314–316 (1979).
Hruby, D. E., Guarino, L. A. & Kates, J. R. J. Virol. 29, 705–715 (1979).
Aloni, Y., Shani, M. & Reuveni, Y. Proc. natn. Acad. Sci. U.S.A. 72, 2587–2591 (1975).
Griffin, B. E., Fried, M. & Cowie, A. Proc. natn. Acad. Sci. U.S.A. 71, 2077–2081 (1974).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 75, 1274–1278 (1972).
Laskey, R. A. & Mills, A. D. Eur. J. Biochem. 56, 335–341 (1975).
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Piper, P., Wardale, J. & Crew, F. Splicing of the late mRNAs of polyoma virus does not occur in the cytoplasm of the infected cell. Nature 282, 686–691 (1979). https://doi.org/10.1038/282686a0
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DOI: https://doi.org/10.1038/282686a0
