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Gene transfer in intact animals

Nature volume 284, pages 422–425 (1980)Cite this article

Abstract

Resistance to methotrexate was induced in bone marrow cells of mice by transformation in vitro with DNA from a drug-resistant cell line. Transformed cells were injected in vivo and haematopoietic cells expressing resistance were selected by drug treatment of recipients. Transformed cells had elevated levels of dihydrofolate reductase and demonstrated a proliferative advantage over untransformed cells, indicating successful gene transfer.

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References

  1. Avery, O. T., Macleod, C. M. & McCarty, M. J. exp. Med. 79, 137 (1944).

    Article  CAS  Google Scholar 

  2. Munyon, W., Kraiselburd, E., Davies, D. & Mann, J. J. Virol. 7, 813–820 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Fournier, R. E. K. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 74, 319–323 (1977).

    Article  ADS  CAS  Google Scholar 

  4. Bachetti, S. & Graham, F. I. Proc. natn. Acad. Sci. U.S.A. 74, 1590–1594 (1977).

    Article  ADS  Google Scholar 

  5. Maitland, N. J. & McDougall, J. K. Cell 11, 233–241 (1977).

    Article  CAS  Google Scholar 

  6. Pellicer, A., Wigler, M., Axel, R. & Silverstein, S. Cell 14, 133–141 (1978).

    Article  CAS  Google Scholar 

  7. Wigler, M., Pellicer, A., Siverstein, S. & Axel, R. Cell 14, 725–731 (1978).

    Article  CAS  Google Scholar 

  8. Osborn, M. J., Freeman, M. & Huennekens, F. M. Proc. Soc. exp. Biol. Med. 97, 429 (1958).

    Article  CAS  Google Scholar 

  9. Ford, C. E. in Tissue Grafting and Radiation (eds Micklem, H. S. & Doutit, J. F.) (Academic, New York, 1966).

    Google Scholar 

  10. Schimke, R. T., Kaufman, R. J., Alt, F. W. & Kellems, R. F. Science 202, 1051–1055 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Kaufman, R.J., Bertino, J. R. & Schimke, R. T. J. biol. Chem. 253, 5852–5860 (1978).

    CAS  PubMed  Google Scholar 

  12. Metcalf, D. & Moore, M. A. S. Haemopoietic Cells, 44–47 (North-Holland, Amsterdam, 1971).

  13. Smith, W. W., Wilson, S. M. & Fred, S. S. J. natn. Cancer Inst. 40, 847–854 (1968).

    CAS  Google Scholar 

  14. Cline, M. J. & Haskell, C. M. Cancer Chemotherapy 3rd edn (Saunders, Philadelphia, 1979).

  15. Kellems, R. E., Morhenn, V. B., Pfendt, E. A., Alt, F. W. & Schimke, R. T. (in preparation).

  16. Till, J. E. & McCulloch, E. A. Radiat. Res. 14, 213 (1961).

    Article  ADS  CAS  Google Scholar 

  17. Hillcoat, B. L., Sweet, V. & Bertino, J. R. Proc. natn. Acad. Sci. U.S.A. 58, 1632 (1967).

    Article  ADS  CAS  Google Scholar 

  18. Hayman, R., McCready, R. & Van Der Weyden, M. Analyt. Biochem. 87, 460 (1978).

    Article  CAS  Google Scholar 

  19. Southern, D. M. J. molec. Biol. 98, 503 (1975).

    Article  CAS  Google Scholar 

  20. Mercola, K. E., Stang, H., Browne, J., Salser, W. & Cline, M. J. Science (submitted).

Download references

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Authors and Affiliations

  1. Department of Medicine, University of California, Los Angeles, California, 90024

    M. J. Cline, H. Stang, K. Mercola, L. Morse & R. Ruprecht

  2. Department of Biology, University of California, Los Angeles, California, 90024

    J. Browne & W. Salser

  3. Molecular Biology Institute, University of California, Los Angeles, California, 90024

    M. J. Cline & W. Salser

Authors
  1. M. J. Cline
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  2. H. Stang
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  3. K. Mercola
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  4. L. Morse
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  5. R. Ruprecht
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  6. J. Browne
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  7. W. Salser
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Cline, M., Stang, H., Mercola, K. et al. Gene transfer in intact animals. Nature 284, 422–425 (1980). https://doi.org/10.1038/284422a0

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