Abstract
Most cells are thought to contain trace amounts of 2,3-diphosphoglycerate (DPG), as it acts as a cofactor in the interconversion of 2-phosphoglycerate and 3-phosphoglycerate by the glycolytic enzyme phosphoglyceromutase. DPG is synthesized from 1,3-diphosphoglycerate by the action of diphosphoglycerate mutase. Lowry et al.1 reported levels of 29 µmol DPG per kg wet weight brain tissue which is approximately 3 pmol per 108 cells, assuming that 1 g of brain tissue contains 109 cells. In contrast, erythroid cells contain 50–100 nmol DPG per 108 cells, depending on the species and the stage of development2. This is of the order of a 1,000-fold more DPG compared with non-erythroid cells. In red cells DPG concentration modulates the binding of oxygen to haemoglobin3,4. I show here that erythroid precurser cells also contain markedly raised levels of DPG.
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Yeoh, G. Levels of 2,3-diphosphoglycerate in Friend leukaemic cells. Nature 285, 108–109 (1980). https://doi.org/10.1038/285108a0
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DOI: https://doi.org/10.1038/285108a0
