Abstract
The mouse mammary gland in serum-free whole organ culture can be manipulated hormonally to undergo one complete physiological cycle consisting of lobuloalveolar development1, functional differentiation and regression2, mimicking processes that occur in vivo. A second cycle has not previously been achieved in vitro. The present study has identified a specific requirement for epidermal growth factor (EGF) in the morphological development of mammary lobuloalveoli, allowing two complete cycles of development and regression in culture.
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References
Ichinose, R. R. & Nandi, S. J. Endocr. 35, 331–340 (1966).
Wood, B. G., Washburn, L. L., Mukherjee, A. S. & Banerjee, M. R. J. Endocr. 65, 1–6 (1975).
Tonelli, Q. J., Custer, R. P. & Sorof, S. Cancer Res. 39, 1784–1792 (1979).
Banerjee, M. R., Wood, B. G., Lin, F. K. & Crump, L. R. Tissue Culture Ass. Manual 2, 457–462 (1976).
Terry, P. M., Ball, E. M., Ganguly, R. & Banerjee, M. R. J. Immun. Meth. 9, 123–134 (1975).
Carpenter, G. & Cohen, S. A. Rev. Biochem. 48, 193–216 (1979).
Byyny, R. L., Orth, D. N., Cohen, S. & Doyne, E. S. Endocrinology 95, 776–782 (1974).
Turkington, R. W. Expl. Cell Res. 57, 79–85 (1969).
Stoker, M. G. P., Pigott, D. & Taylor-Papadimitriou, J. Nature 264, 764–767 (1976).
Turkington, R. W. Cancer Res. 29, 1457–1458 (1969).
Rudland, P. S., Hallowes, R. C., Durbin, H. & Lewis, D. J. Cell Biol. 73, 561–577 (1977).
Dickens, M. S., Custer, R. P. & Sorof, S. Proc. natn. Acad. Sci. U.S.A. 76, 5891–5895 (1979).
Telang, N. T., Banerjee, M. R., Iyer, A. P. & Kundu, A. B. Proc. natn. Acad. Sci. U.S.A. 76, 5886–5890 (1979).
Banerjee, M. R., Wood, B. G. & Washburn, L. L. J. natn. Cancer Inst. 53, 1387–1393 (1974).
Todaro, G. J. & De Larco, J. E. Cancer Res. 38, 4147–4154 (1978).
Todaro, G. J., De Larco, J. E. & Sporn, M. B. Nature 276, 272–274 (1978).
De Larco, J. E. & Todaro, G. J. Proc. natn. Acad. Sci. U.S.A. 75, 4001–4005 (1978).
Shoyab, M., De Larco, J. E. & Todaro, G. J. Nature 279, 387–391 (1979).
Lee, L.-S. & Weinstein, I. B. Science 202, 313–315 (1978).
Cherington, P. V., Smith, B. L. & Pardee, A. B. Proc. natn. Acad. Sci. U.S.A. 76, 3937–3941 (1979).
Banerjee, M. R. & Rodgers, F. M. J. Endocr. 49, 39–49 (1971).
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Tonelli, Q., Sorof, S. Epidermal growth factor requirement for development of cultured mammary gland. Nature 285, 250–252 (1980). https://doi.org/10.1038/285250a0
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DOI: https://doi.org/10.1038/285250a0
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