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Epidermal growth factor requirement for development of cultured mammary gland

Abstract

The mouse mammary gland in serum-free whole organ culture can be manipulated hormonally to undergo one complete physiological cycle consisting of lobuloalveolar development1, functional differentiation and regression2, mimicking processes that occur in vivo. A second cycle has not previously been achieved in vitro. The present study has identified a specific requirement for epidermal growth factor (EGF) in the morphological development of mammary lobuloalveoli, allowing two complete cycles of development and regression in culture.

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Tonelli, Q., Sorof, S. Epidermal growth factor requirement for development of cultured mammary gland. Nature 285, 250–252 (1980). https://doi.org/10.1038/285250a0

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