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The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I

Nature volume 286, pages 854–860 (1980)Cite this article

Abstract

Many specific sites in Drosophila chromatin are hypersensitive to DNase I. The positions of such sites were mapped along the regions of the genome coding for two heat shock proteins. Such sites lie at the 5′ ends of heat shock genes and may function as elements for recognition by molecules which regulate gene activity.

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References

  1. Felsenfeld, G. Nature 271, 115–122 (1978).

    Article  ADS  CAS  Google Scholar 

  2. Wu, C., Bingham, P. M., Livak, K. J., Holmgren, R. & Elgin, S. C. R. Cell 16, 797–806 (1979).

    Article  CAS  Google Scholar 

  3. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  4. Schneider, I. J. Embryol. exp. Morph. 27, 353–365 (1972).

    CAS  PubMed  Google Scholar 

  5. Ashburner, M. & Bonner, J. J. Cell 17, 214–254 (1979).

    Article  Google Scholar 

  6. Ritossa, F. Experientia 18, 571–573 (1962).

    Article  CAS  Google Scholar 

  7. Ashburner, M. Chromosoma 31, 356–376 (1970).

    Article  CAS  Google Scholar 

  8. Tissieres, A., Mitchell, H. K. & Tracy, U. M. J. molec. Biol. 84, 389–398 (1974).

    Article  CAS  Google Scholar 

  9. McKenzie, S. L., Henikoff, S. & Meselson, M. Proc. natn. Acad. Sci. U.S.A. 72, 1117–1121 (1975).

    Article  ADS  CAS  Google Scholar 

  10. Spradling, A., Penman, S. & Pardue, M. L. Cell 4, 395–404 (1975).

    Article  CAS  Google Scholar 

  11. Lewis, M., Helmsing, P. J. & Ashburner, M. Proc. natn. Acad. Sci. U.S.A. 72, 3604–3608 (1975).

    Article  ADS  CAS  Google Scholar 

  12. Spradling, A., Pardue, M. L. & Penman, S. J. molec. Biol. 109, 559–587 (1977).

    Article  CAS  Google Scholar 

  13. McKenzie, S. L. & Meselson, M. J. molec. Biol. 117, 279–283 (1977).

    Article  CAS  Google Scholar 

  14. Mirault, M. E., Goldschmidt-Clermont, M., Moran, L., Arrigo, A. P. & Tissieres, A. Cold Spring Harb. Symp. quant. Biol. 42, 819–829 (1978).

    Article  CAS  Google Scholar 

  15. Henikoff, S. & Meselson, M. Cell 12, 441–451 (1977).

    Article  CAS  Google Scholar 

  16. Ish-Horowicz, D., Holden, J. J. & Gehring, W. J. Cell 12, 643–652 (1977).

    Article  CAS  Google Scholar 

  17. Livak, K. J., Freund, R., Schweber, M., Wensink, P. C. & Meselson, M. Proc. natn. Acad. Sci. U.S.A. 75, 5613–5617 (1978).

    Article  ADS  CAS  Google Scholar 

  18. Schedl, P. et al. Cell 14, 921–929 (1978).

    Article  CAS  Google Scholar 

  19. Craig, E. A., McCarthy, B. J. & Wadsworth, S. C. Cell 16, 575–588 (1979).

    Article  CAS  Google Scholar 

  20. Moran, L. et al. Cell 17, 1–8 (1979).

    Article  CAS  Google Scholar 

  21. Artavanis-Tsakonas, S., Schedl, P., Mirault, M. E., Moran, L. & Lis, J. Cell 17, 9–18 (1979).

    Article  CAS  Google Scholar 

  22. Mirault, M. E., Goldschmidt-Clermont, M., Artavanis-Tsakonas, S. & Schedl, P. Proc. natn. Acad. Sci. U.S.A. 76, 5254–5258 (1979).

    Article  ADS  CAS  Google Scholar 

  23. Ish-Horowicz, D., Pinchin, S. M., Schedl, P., Artavanis-Tsakonas, S. & Mirault, M. E. Cell 18, 1351–1358 (1979).

    Article  CAS  Google Scholar 

  24. Holmgren, R., Livak, K., Morimoto, R., Freund, R. & Meselson, M. Cell 18, 1359–1370 (1979).

    Article  CAS  Google Scholar 

  25. Goldschmidt-Clermont, M. Nucleic Acids Res. 8, 235–251 (1980).

    Article  CAS  Google Scholar 

  26. Bernardi, A., Gaillard, C. & Bernardi, G. Eur. J. Biochem. 52, 451–457 (1975).

    Article  CAS  Google Scholar 

  27. Lis, J. T., Prestidge, L. & Hogness, D. S. Cell 14, 901–919 (1978).

    Article  CAS  Google Scholar 

  28. Wu, C., Wong, Y. C. & Elgin, S. C. R. Cell 16, 807–814 (1979).

    Article  CAS  Google Scholar 

  29. Wensink, P. C., Finnegan, D. J., Donelson, J. E. & Hogness, D. S. Cell 3, 315–325 (1974).

    Article  CAS  Google Scholar 

  30. Wu, C. thesis, Harvard Univ. (1979).

  31. Anfinsen, C. B., Cuatrecasas, P. & Taniuchi, H. in The Enzymes Vol. IV, 3rd edn (ed. Boyer, P. D.) 177–199 (Academic, New York, 1971).

    Google Scholar 

  32. Ponder, B. A. & Crawford, C. V. Cell 11, 35–49 (1977).

    Article  CAS  Google Scholar 

  33. Musich, P. R., Maio, J. J. & Brown, F. L. J. molec. Biol. 117, 657–677 (1977).

    Article  CAS  Google Scholar 

  34. Chao, M. V., Gralla, J. & Martinson, H. G. Biochemistry 8, 1068–1074 (1979).

    Article  Google Scholar 

  35. Wittig, B. & Wittig, S. Cell 18, 1173–1183 (1979).

    Article  CAS  Google Scholar 

  36. Fittler, F. & Zachau, H. G. Nucleic Acids Res. 7, 1–13 (1979).

    Article  CAS  Google Scholar 

  37. Scott, W. A. & Wigmore, D. J. Cell 15, 1511–1518 (1978).

    Article  CAS  Google Scholar 

  38. Varshavsky, A. J., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).

    Article  CAS  Google Scholar 

  39. Waldeck, W., Fohring, B., Chowdhury, K., Gruss, P. & Sauer, G. Proc. natn. Acad. Sci. U.S.A. 75, 5964–5968 (1978).

    Article  ADS  CAS  Google Scholar 

  40. Kuo, M. T., Mandel, J. L. & Chambon, P. Nucleic Acids Res. 7, 2105–2113 (1979).

    Article  CAS  Google Scholar 

  41. Hayward, G. S. & Smith, M. G. J. molec. Biol. 63, 383–395 (1972).

    Article  CAS  Google Scholar 

  42. Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).

    Article  ADS  CAS  Google Scholar 

  43. Denhardt, D. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

    Article  CAS  Google Scholar 

  44. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).

    Article  CAS  Google Scholar 

Download references

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  1. Society of Fellows, Harvard University, and The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts, 02138

    Carl Wu

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  1. Carl Wu
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Wu, C. The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature 286, 854–860 (1980). https://doi.org/10.1038/286854a0

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