Abstract
A membrane receptor protein for asialoglycoproteins induces voltage-dependent increases in ion conductance across a lipid bilayer, probably reflecting penetration of the protein into the bilayer towards an electrically positive pole. In the presence of specific ligand for the receptor, this penetration leads to a ‘translocation’ of the receptor from one side of the bilayer to the other. These observations suggest a mechanism by which biological membranes might regulate the disposition of their proteins, and a way in which membrane receptors involved in endocytosis might be spared lysosomal destruction in order to be recycled to the plasma membrane.
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References
Rothman, J. E. & Lenard, J. Science 195, 743–753 (1977).
Op den Kamp, J. A. F. A. Rev. Biochem. 48, 47–71 (1979).
Ashwell, G. & Morell, A G. Adv. Enzym. 41, 99–128 (1974).
Tanabe, T., Pricer, W. E. Jr & Ashwell, G. J. biol. Chem. 254, 1038–1043 (1979).
Kawasaki, T. & Ashwell, G. J. biol. Chem. 251, 1296–1302 (1976).
Klausner, R. D. et al. Proc. natn. Acad. Sci. U.S.A. 77, 5087–5091 (1980).
Blumenthal, R. & Shamoo, A. E. in The Receptors Vol. 1 (ed. O'Brien, R. D.) 215–245 (Plenum, New York, 1979).
Haydon, D. A. & Hladky, S. B. Q. Rev. Biophys. 5, 187–282 (1972).
Shinitzky, M. in Physical–Chemical Aspects of Cell Surface Events in Cellular Regulation (eds DeLisi, C. & Blumenthal, R.) 173–181 (Elsevier, New York, 1979).
Tien, H. Bilayer Lipid Membranes (BLM) Theory and Practice (Dekker, New York, 1974).
Somlyo, A. P., Somlyo, A. V. & Friedmann, N. Ann. N.Y. Acad. Sci. 185, 108–114 (1971).
Goldman, R. & Rottenberg, H. FEBS Lett. 33, 233–238 (1973).
Henning, R. Biochim. biophys. Acta 401, 307–316 (1975).
Ehrenstein, G. & Lecar, H. Q. Rev. Biophys. 10, 1–344 (1977).
Finkelstein, A., Rubin, L. L. & Tzeng, M. Science 193, 1009–1011 (1976).
Schein, S. J., Kagan, B. L. & Finkelstein, A. Nature 276, 159–163 (1978).
Blumenthal, R. & Shamoo, A. E. J. Membrane Biol. 19, 141–162 (1974).
Schein, S. J., Colombini, M. & Finkelstein, A. J. Membrane Biol. 30, 99–120 (1976).
Michaels, D. W., Abramovitz, A. S., Hammer, C. H. & Mayer, M. M. Proc. natn. Acad. Sci. U.S.A. 73, 2652–2856 (1976).
Henkart, P. & Blumenthal, R. Proc. natn. Acad. Sci. U.S.A. 72, 2789–2793 (1975).
Hodgkin, A. L. & Huxley, A. F. J. Physiol., Lond. 117, 500–544 (1952).
Miller, C. & Rosenberg, R. L. J. gen. Physiol. 74, 457–478 (1979).
Date, T., Zwizinsky, C., Ludmerer, S. & Wickner, W. Proc. natn. Acad. Sci. U.S.A. 77, 825–831 (1980).
Mueller, P., Rudin, D. O., Tien, H. T. & Wescott, W. C. Nature 194, 979–981 (1962).
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Blumenthal, R., Klausner, R. & Weinstein, J. Voltage-dependent translocation of the asialoglycoprotein receptor across lipid membranes. Nature 288, 333–338 (1980). https://doi.org/10.1038/288333a0
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DOI: https://doi.org/10.1038/288333a0
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