Abstract
To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, we have constructed several deletion mutants in the promoter region. The TATA box region is apparently involved infixing initiation precisely within a narrow area, but is dispensable for gene expression, while the sequences located more than 150 base pairs upstream are indispensable.
Similar content being viewed by others
Article PDF
References
Breathnach, R. & Chambon, P. A. Rev. Biochem. (in the press).
Corden, J. et al. Science 209, 1406–1414 (1980).
Ziff, E. & Evans, R. Cell 15, 1463–1475 (1978).
Flavell, A., Cowie, A., Arrand, J. & Kamen, R. J. Virol. 33, 902–908 (1980).
Haegeman, G. & Fliers, W. Nucleic Acids Res. 5, 2359–2371 (1978).
Baker, C., Herisse, J., Courtois, G., Galibert, F. & Ziff, E. Cell 18, 569–580 (1979).
Groner, Y., Carmi, P. & Aloni, Y. Nucleic Acids Res. 4, 3959–3968 (1978).
Ghosh, P., Reddy, V., Swinscoe, J., Lebowitz, P. & Weissman, S. J. molec. Biol. 126, 813–846 (1979).
Weil, P., Luse, D., Segall, J. & Roeder, R. Cell 18, 469–484 (1979).
Manley, J., Fire, A., Cano, A., Sharp, P. & Gefter, M. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).
Wasylyk, B., Kedinger, C., Corden, J., Brison, O. & Chambon, P. Nature 285, 367–373 (1980).
Wasylyk, B. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Benoist, C. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 3865–3869 (1980).
Gluzman, Y., Sambrook, J. & Frisque, R. Proc. natn. Acad. Sci. U.S.A. 77, 3898–3902 (1980).
Mueller, C., Graessmann, A. & Graessmann, M. Cell 15, 579–586 (1978).
Grosschedl, R. & Birnstiel, M. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).
Acheson, N. in Molecular Biology of Tumor Viruses Pt 2 (ed. Tooze, J.) 125–204 (Cold Spring Harbor, New York, 1980).
Thompson, J., Radonovich, M. & Salzman, N. J. Virol. 31, 437–446 (1979).
Reddy, V., Ghosh, P., Lebowitz, P., Piatak, M. & Weissman, S. J. Virol. 30, 279–296 (1979).
Haegeman, G. & Fiers, W. J. Virol. 35, 955–961 (1980).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).
Alwine, J., Kemp, D. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Weaver, R. & Weissmann, C. Nucleic Acids Res. 7, 1175–1193 (1979).
Mathis, D. J. & Chambon, P. Nature 290, 310–315 (1981).
Reed, S., Stark, G. & Alwine, J. Proc. natn. Acad. Sci. U.S.A. 73, 3083–3087 (1976).
Tjian, R. Cell 13, 165–179 (1978).
Shalloway, D., Kleinberger, T. & Livingston, D. Cell 20, 411–422 (1980).
Myers, R. and Tjian, R. Proc. natn. Acad. Sci. U.S.A. 77, 6491–6495 (1980).
Van Heuverswyn, H. & Fiers, W. Eur. J. Biochem. 100, 51–60 (1979).
Scott, W. & Wigmore, D. Cell 15, 1511–1518 (1978).
Sundin, O. & Varshavsky, A. J. molec. Biol. 132, 535–546 (1979).
Varshavsky, A., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).
Saragosti, M., Moyne, G. & Yaniv, M. Cell 20, 65–73 (1980).
Wigmore, D., Eaton, R. & Scott, W. Virology 104, 462–473 (1980).
Stalder, J. et al. Cell 20, 451–460 (1980).
Wu, C. Nature 286, 854–860 (1980).
Flavell, R. Nature 285, 356–357 (1980).
Mathis, D., Oudet, P. & Chambon, P. Prog. Nucleic Acids Res. molec. Biol. 24, 1–55 (1980).
Mertz, J. & Berg, P. Virology 62, 112–124 (1974).
Wigler, M., Pellicer, A., Silverstein, S. & Axel, R. Cell 14, 725–731 (1978).
Reddy, V. et al. Science 200, 494–502 (1978).
Legerski, R., Hodnett, J. & Gray, H. Nucleic Acids Res. 5, 1445–1464 (1978).
Fiers, W. et al. Nature 273, 113–120 (1978).
Sanger, F. & Coulson, A. FEBS Lett. 87, 107–110 (1977).
Favaloro, J., Treisman, R. & Kamen, R. Meth. Enzym. 65, 718–749 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benoist, C., Chambon, P. In vivo sequence requirements of the SV40 early promoter region. Nature 290, 304–310 (1981). https://doi.org/10.1038/290304a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/290304a0
This article is cited by
-
Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues
Microbial Cell Factories (2022)
-
Organization and regulation of gene transcription
Nature (2019)
-
Scarless genome editing: progress towards understanding genotype–phenotype relationships
Current Genetics (2018)
-
Self-cloning brewing yeast: a new dimension in beverage production
European Food Research and Technology (2013)
-
Histone modification pattern evolution after yeast gene duplication
BMC Evolutionary Biology (2012)
