Abstract
The advantages of using monoclonal antibodies account for current interest in antibodies specific for interferem1–3. The successful isolation of a hybridoma line secreting mouse monoclonal antibodies to human leukocyte interf eron (IFN-α) has already been reported4. We have now established a stable hybrid myeloma line (FO) which secretes antibody against human fibroblast interferon (IFN-β). We have demonstrated that, even after removal of much of the sugar moiety of IFN-β, the monoclonal anti-IFN-β antibodies neutralize the antiviral activity of human IFN-β but not that of human IFN-α. We suggest the possible use of these monoclonal antibodies for the identification and purification of IFN-β expressed by DNA cloned in bacteria.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).
Köhler, G. & Milstein, C. Eur. J. Immun. 6, 511–519 (1976).
Köhler, G., Hengartner, H. & Shulman, M. J. Eur. J. Immun. 8, 82–88 (1978).
Secher, D. S. & Burke, D. C. Nature 285, 446–450 (1980).
Menge, U. & Kula, M. R. German patent application No. P 2943016.8.
Havell, E. A. & Vilcek, J. Antimicrob. Agents Chemother. 2, 476–484 (1972).
de Fazekas, S. E., & Scheidegger, S. J. immun, meth. 35, 1–25 (1980).
Towbin, H., Staehelin, T. & Gordon, J. Proc. natn, Acad, Sci. U.S.A. 76, 4350–4354 (1979).
Laemmli, U. K. Nature 227, 680–685 (1970).
Weissenbach, J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7152–7156 (1980).
Sagar, A. D., Pickering, L. A., Sussman-Berger, P., Stewart, W. E. Pickering, L. A., Sussman-Berger, P., Stewart, W. E. II & Sehgal, P. B. Nucleic Acids Res. 9, 149–160 (1981).
Bowen, B., Steinberg, J., Laemmli, U. K. & Weintraub, H. Nucleic Acids Res. 8, 1–19 (1980).
Hunter, W. M. & Greenwood, I. C. Nature 194, 495–496 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hochkeppel, H., Menge, U. & Collins, J. Monoclonal antibodies against human fibroblast interferon. Nature 291, 500–501 (1981). https://doi.org/10.1038/291500a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/291500a0
