Abstract
Two key steps in meiotic maturation of the Xenopus oocyte involve protein phosphorylation–dephosphorylation1. A burst of cyclic AMP-independent phosphorylation occurs at the time of the breakdown of the nuclear envelope, whether maturation is triggered by progesterone, by the inhibitor of cyclic AMP-dependent protein kinase (PKI)2 or by the maturation promoting factor (MPF)1,3. Also, an in ovo decrease in the level of the free catalytic (C) subunit of cyclic AMP-dependent protein kinase, induced by microinjection of pure PKI4, initiates meiotic maturation1,2,4. This suggests that MPF appears only after dephosphorylation of a phosphorylated maturation protein Mp-P4. We now show, by microinjection of pure inhibitor-1 of protein phosphatase-15, which blocks progesterone- and PKI-induced maturation but not the MPF-induced one, that protein phosphatase-1 catalyses the dephosphorylation of Mp-P.
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Huchon, D., Ozon, R. & Demaille, J. Protein phosphatase-1 is involved in Xenopus oocyte maturation. Nature 294, 358–359 (1981). https://doi.org/10.1038/294358a0
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DOI: https://doi.org/10.1038/294358a0
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