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Reversible induction of natural killer cell activity in cloned murine cytotoxic T lymphocytes

Nature volume 305, pages 155–158 (1983)Cite this article

Abstract

Natural killer (NK) activity is a poorly understood component of the immune system, generally identified as the ability to kill certain tumour cells1,2. Perhaps the most controversial issue has been the lineage to which cells displaying this activity belong. Extensive studies of surface antigens on cells with NK activity in both mouse and man have led to enigmatic results, such cells apparently bearing markers of both T-cell (Thy-1 and E receptor) and myeloid (Mac-1 and OKM1) lineages3–6. A fresh approach to this problem would be to take cells of known lineage and test whether they express, or could be induced to express, NK cell function. Using this approach we show here that monoclonal cytotoxic T lymphocyte (CTL) lines can be induced, by culture in high concentrations of spleen cell supernatant, to express a new lytic activity apparently identical with that of splenic cells NK activity. Preliminary evidence implicates both interleukin-2(IL-2) and interferon (IFN) as mediators of this phenomenon. These findings clearly demonstrate that cells of T cell lineage have the capacity to express NK activity.

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References

  1. Herberman, R. B. & Holden, H. T. Adv. Cancer Res. 27, 305–377 (1978).

    Article  CAS  PubMed  Google Scholar 

  2. Kiessling, R. & Haller, O. Contemp. Top. Immunobiol. 8, 171–225 (1978).

    Article  CAS  PubMed  Google Scholar 

  3. Herberman, R. B., Nunn, M. E. & Holden, H. T. J. Immun. 121, 304–309 (1978).

    CAS  PubMed  Google Scholar 

  4. West, W. M., Cannon, G. B. & Herberman, R. B. J. Immun. 118, 355–361 (1977).

    CAS  PubMed  Google Scholar 

  5. Holmberg, L. A., Springer, T. A. & Ault, K. A. J. Immun. 127, 1792–1799 (1981).

    CAS  PubMed  Google Scholar 

  6. Zarling, J. M. & Kung, P. C. Nature 288, 394–396 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Glasebrook, A. L. & Fitch, F. W. J. exp. Med. 151, 876–895 (1980).

    Article  CAS  PubMed  Google Scholar 

  8. Bonavida, B. & Bradley, T. P. Transplantation 21, 94–102 (1976).

    Article  CAS  PubMed  Google Scholar 

  9. Sitkovsky, M. V., Pasternak, M. S. & Eisen, M. J. Immun. 129, 1372–1376 (1982).

    CAS  PubMed  Google Scholar 

  10. Gidlund, M., Orn, A., Wigzell, H., Senik, A. & Gresser, I. Nature 273, 273, 759–761 (1978).

    Article  Google Scholar 

  11. Henney, C. S., Kuribayashi, K., Kern, D. E. & Gillis, S. Nature 291, 335–338 (1982).

    Article  ADS  Google Scholar 

  12. Dennert, G., Yogeeswaran, G. & Yamagata, S. J. exp. Med. 153, 544–556 (1981).

    Article  Google Scholar 

  13. Nabel, G., Bucalo, L. R., Allard, J., Wigzell, H. & Cantor, H. J. exp. Med. 153, 1582–1591 (1981).

    Article  CAS  PubMed  Google Scholar 

  14. Brooks, C. G., Kuribayashi, K., Sale, G. E. & Henney, C. S. J. Immun. 128, 2326–2335 (1982).

    CAS  PubMed  Google Scholar 

  15. Handa, K., Suzuki, R., Matsui, H., Simizu, Y. & Kumagai, K. J. Immun. 130, 988–992 (1983).

    CAS  PubMed  Google Scholar 

  16. Brooks, C. G., Urdal, D. & Henney, C. S., Immun. Rev. 72, 43–72 (1983).

    Article  CAS  PubMed  Google Scholar 

  17. Klein, E. Immun. Today. 1, iv–vi (1980).

    Google Scholar 

  18. Brooks, C. G., Burton, R. C., Pollack, S. B. & Henney, C. S. J. Immun. 131 (in the press).

  19. Click, R. E., Benck, L. & Alter, B. J. Cell. Immun. 3, 264–276 (1972).

    Article  CAS  Google Scholar 

  20. Cikes, M., Friberg, S. & Klein, G. J. natn. Cancer Inst. 50, 347–362 (1973).

    Article  CAS  Google Scholar 

  21. Durdik, J. M., Beck, B. N., Clark, E. A. & Henney, C. S., J. Immun. 125, 683–688 (1980).

    CAS  PubMed  Google Scholar 

  22. Dunn, T. B. & Potter, M. J. J. natn. Cancer Inst. 180, 587–603 (1957).

    Google Scholar 

  23. Gorer, P. A. Br. J. Cancer. 4, 372–392 (1950).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Gillis, et al. Immun. Rev. 63, 167–208 (1982).

    Article  CAS  PubMed  Google Scholar 

Download references

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  1. Program in Basic Immunology, Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104, USA

    Colin G. Brooks

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  1. Colin G. Brooks
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Brooks, C. Reversible induction of natural killer cell activity in cloned murine cytotoxic T lymphocytes. Nature 305, 155–158 (1983). https://doi.org/10.1038/305155a0

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