Abstract
Members of the geminivirus group are characterized by their twinned virions which encapsidate a genome of circular, single-stranded DNA1. It has been suggested that the genomes of some members, specifically bean golden mosaic virus2 and tomato golden mosaic virus3,4 are bipartite. Recently, the complete nucleotide sequences of two similar-sized, circular DNA molecules (DNAs 1 and 2) from cassava latent virus (CLV) have been determined in this laboratory5. There has been no unequivocal evidence, however, to show that two such DNA molecules represent the infective unit of the virus genome. Here I describe the construction of recombinant DNA clones that contain full-length copies of CLV DNAs 1 and 2 inserted into bacteriophage M13. The inserts, on excision from the cloning vectors, are together shown to be infectious in Nicotiana benthamiana, verifying the bipartite nature of the genome.
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Stanley, J. Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305, 643–645 (1983). https://doi.org/10.1038/305643a0
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DOI: https://doi.org/10.1038/305643a0
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