Abstract
Nucleotide sequence and deletion analysis have been used to identify the regulatory and coding sequences comprising the cholera toxin operon (ctx). Incorporation of defined in vitro-generated ctx deletion mutations into Vibrio cholerae by in vivo genetic recombination produced strains which have practical value in cholera vaccine development.
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Mekalanos, J., Swartz, D., Pearson, G. et al. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature 306, 551–557 (1983). https://doi.org/10.1038/306551a0
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DOI: https://doi.org/10.1038/306551a0
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