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Relationship between an enhancer element in the human antithrombin III gene and an immunoglobulin light-chain gene enhancer

Nature volume 316, pages 845–848 (1985)Cite this article

Abstract

Enhancers, cis-acting transcriptional control elements have been described in both viral and cellular genes1. They influence transcription in a quantitative fashion, act over relatively large distances (several kilobases, kb) and behave independently of their position and orientation. Enhancers have been described in immunoglobulin, chymotrypsin and insulin genes2–9. They bear little homology with each other except for an 8-base pair (bp) 'consensus' core element, GTGGAAATTTG (refs 10, 11), but even this element is sometimes non-homologous9.I have searched for such elements in the human antithrombin HI (AT-III) gene. AT-III is an important coagulation protein which inactivates thrombin12. It is produced by the liver and, to a lesser extent, by the kidney13. Here, I report that the 5' flanking region of the AT-III gene encodes a segment homolgous with the enhancer containing the joining–constantκ (Jκ–Cκ) intron of immunoglobulin κ-chain genes. This extensive homology suggests the existence of regulatory factors that recognize common DNA sequences in lymphoid tissues and in those which express AT-III.

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Authors and Affiliations

  1. Section of Hematology/Oncology, Department of Pediatrics, C. S. Mott Children's Hospital and the Committee on Cellular and Molecular Biology, University of Michigan School of Medicine, Ann Arbor, Michigan, 48109, USA

    Edward V. Prochownik

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  1. Edward V. Prochownik
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Prochownik, E. Relationship between an enhancer element in the human antithrombin III gene and an immunoglobulin light-chain gene enhancer. Nature 316, 845–848 (1985). https://doi.org/10.1038/316845a0

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