Abstract
In eukaryotes the transcriptional control of RNA polymerase II-mediated gene expression is exerted by cis-acting regulatory DNA elements classified as promoter and enhancer sequences. These elements are composed of a number of different protein binding sites (see refs 1, 2 for review). The regulatory factors that recognize such 'modules' may be ubiquitous, tissue- or stage-specific, and positively or negatively acting. According to this model the transcriptional activity of a given gene is programmed by a combination of different modules. We analysed such a site of protein-DN A interaction, the octamer motif3–6, in the enhancers of the simian virus (SV40) early genes and the murine immunoglobulin heavy-chain gene, and in the distal sequence element (DSE) of the U2 small nuclear (sn)RNA gene of Xenopus laevis. The corresponding DNA-binding factor appears to be the same in the three cases. Moreover, a fraction containing partially purified octamer motif binding factor has a stimulatory effect on transcription in an in vitro system.
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Bohmann, D., Keller, W., Dale, T. et al. A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes. Nature 325, 268–272 (1987). https://doi.org/10.1038/325268a0
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DOI: https://doi.org/10.1038/325268a0
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