Abstract
The crystal structure of a specific complex between the DNA-binding domain of phage 434 represser and a synthetic 434 operator DNA shows interactions that determine sequence-dependent affinity. The represser recognizes its operators by its complementarity to a particular DNA conformation as well as by direct interaction with base pairs in the major groove.
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References
Ptashne, M. A Genetic Switch (Cell Press, Cambridge, Massachusetts, 1986).
Koudelka, J., Harrison, S.C. & Ptashne, M. Nature 326, 886–888 (1987).
Wharton, R. thesis, Harvard Univ. (1985).
Anderson, J., Ptashne, M. & Harrison, S. C. Nature 316, 596–601 (1985).
Pabo, C. O. & Lewis, M. Nature 298, 443–447 (1982).
Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754–758 (1981).
McKay, D. B. & Steitz, T. A. Nature 290, 744–749 (1981).
Schevitz, R. W., Otwinowski, Z., Joachimiak, A., Lawson, C. L. & Sigler, P. B. Nature 317, 782–786 (1985).
Pabo, C. O. & Sauer, R. A. Rev. Biochem. 53, 293–321 (1984).
Anderson, J., Ptashne, M. & Harrison, S. C. Proc. natn. Acad. Sci. U.S.A. 181, 1307–1311 (1984).
Rossmann, M. G. & Blow, D. M. Acta cryslallogr. 16, 39–45 (1973).
Bricogne, G. Acta crystallogr. A13O, 395–405 (1974).
Bricogne, G. Acta crystallogr. A132, 832–847 (1976).
Hogle, J., Chow, M. & Filman, D. Science 229, 1359 (1985).
Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).
Hendrickson, W. A. & Konnert, J. H. Biomolecular Structure, Conformation, Function and Evolution Vol. I (ed. Srinivasan, R.) 43–57 (Pergamon, Oxford, 1981).
Sussmann, J., Holbrook, S. R., Church, G. M. & Kim, S.-H. Acta crystallogr. 33, 800–804 (1977).
Ten Eyck, L. Acta. crystallogr. A33, 486–492 (1977).
Hogle, J., Maeda, A. & Harrison, S. C. J. molec. Biol. 191, 625–638 (1986).
Wilson, I. A., Wiley, D. C. & Skehel, J. J. Nature 289, 373–378 (1981).
Drew, H. R. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2179–2183 (1981).
Bushman, R., Anderson, J. E., Harrison, S. C. & Ptashne, M. Nature 316, 651–653 (1985).
Hol, W. G. J., van Duijnen, P. T. & Berendsen, H. J. C. Nature 273, 443–446 (1978).
Pflugrath, J. W. & Quiocho, F. A. Nature 314, 257–260 (1985).
Dreusicke, D. & Schulz, G. FEBS Lett. 208, 301–304 (1986).
Seeman, N. C., Rosenberg, J. N. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 173, 804–808 (1976).
Baker, E. N. & Hubbard, R. E. Progr. Biophys. molec. Biol. 144, 97–179 (1984).
Wharton, R. & Ptashne, M. Nature 326, 888–891 (1987).
Drew, H. R. & Travers, A. A. Cell 37, 491–502 (1984).
Ryder, K., Silver, S., DeLucia, A. L., Fanning, E. & Tegtmeyer, P. Cell 44, 719–725 (1986).
McClarin, J. A. et al. Science 234, 1526–1541 (1986).
Dickerson, R. E. J. molec. Biol. 166, 419–441 (1983).
Record, M. T., deHaseth, P. L. & Lohman, T. M. Biochemistry 16, 4791–4796 (1977).
Crawford, J. thesis, Harvard Univ. (1977).
Durbin, R. et al. Science 232, 1127–1132 (1986).
Kabsch, W. Acta crystallogr. A 33, 922 (1976).
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Anderson, J., Ptashne, M. & Harrison, S. Structure of the represser–operator complex of bacteriophage 434. Nature 326, 846–852 (1987). https://doi.org/10.1038/326846a0
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DOI: https://doi.org/10.1038/326846a0
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