Figure 3
From: Direct transfection and activation of human cutaneous dendritic cells
Quantitative analysis of epidermal LC migration from complete skin samples after biolistic delivery of gold particles or naked DNA. The number of CD1a+ LC remaining in the epidermis 1, 3, 6, 12, 24 and 48 h after biolistic delivery was quantified and compared with the total LC population at time 0 and with the basal migration from untreated skin samples. For each different condition, 12 representative fields were analyzed by fluorescence microscopy at ×400. Means ± 1 s.d. of different treatments from five independent experiments were compared by ANOVA and ad hoc Student–Newman Keules tests.
